Protein Info for MIT1002_03557 in Alteromonas macleodii MIT1002

Annotation: dTDP-glucose 4,6-dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 4 to 183 (180 residues), 50.9 bits, E=4.3e-17
• TIGR01181: dTDP-glucose 4,6-dehydratase
  • amino acids 5 to 346 (342 residues), 511.3 bits, E=4.7e-158
• PF01370: Epimerase
  • amino acids 5 to 261 (257 residues), 235.4 bits, E=2.2e-73
• PF02719: Polysacc_synt_2
  • amino acids 5 to 115 (111 residues), 51.5 bits, E=3e-17
• PF16363: GDP_Man_Dehyd
  • amino acids 6 to 332 (327 residues), 307.4 bits, E=5.6e-95
• PF01073: 3Beta_HSD
  • amino acids 6 to 249 (244 residues), 58.8 bits, E=1.6e-19
• PF07993: NAD_binding_4
  • amino acids 73 to 198 (126 residues), 34.7 bits, E=3.9e-12

Best Hits

Swiss-Prot: 76% identical to RMLB_SHIFL: dTDP-glucose 4,6-dehydratase (rfbB) from Shigella flexneri

KEGG orthology group: K01710, dTDP-glucose 4,6-dehydratase [EC: 4.2.1.46] (inferred from 98% identity to amc:MADE_00991)

MetaCyc: 76% identical to dTDP-glucose 4,6-dehydratase 1 (Escherichia coli K-12 substr. MG1655)
dTDP-glucose 4,6-dehydratase. [EC: 4.2.1.46]

Predicted SEED Role

"dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 4.2.1.46)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• dTDP-4-O-demethyl-β-L-noviose biosynthesis (3/5 steps found)
• dTDP-β-D-fucofuranose biosynthesis (2/4 steps found)
• dTDP-6-deoxy-α-D-allose biosynthesis (2/4 steps found)
• dTDP-N-acetylthomosamine biosynthesis (2/4 steps found)
• dTDP-N-acetylviosamine biosynthesis (2/4 steps found)
• dTDP-L-daunosamine biosynthesis (3/6 steps found)
• dTDP-sibirosamine biosynthesis (3/6 steps found)
• dTDP-α-D-mycaminose biosynthesis (2/5 steps found)
• dTDP-3-acetamido-α-D-fucose biosynthesis (2/5 steps found)
• dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis (2/5 steps found)
• dTDP-β-L-digitoxose biosynthesis (3/7 steps found)
• dTDP-β-L-olivose biosynthesis (3/7 steps found)
• dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis (2/6 steps found)
• dTDP-D-desosamine biosynthesis (2/6 steps found)
• dTDP-β-L-4-epi-vancosamine biosynthesis (3/8 steps found)
• dTDP-β-L-megosamine biosynthesis (3/8 steps found)
• dTDP-β-L-mycarose biosynthesis (2/7 steps found)
• dTDP-α-D-forosamine biosynthesis (3/9 steps found)
• dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis (3/9 steps found)
• superpathway of enterobacterial common antigen biosynthesis (3/10 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (6/19 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)
• superpathway of erythromycin biosynthesis (2/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• superpathway of megalomicin A biosynthesis (3/22 steps found)
• superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis (3/23 steps found)

KEGG Metabolic Maps

• Biosynthesis of vancomycin group antibiotics
• Polyketide sugar unit biosynthesis
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.46

Use Curated BLAST to search for 4.2.1.46

Protein Sequence (360 amino acids)

>MIT1002_03557 dTDP-glucose 4,6-dehydratase (Alteromonas macleodii MIT1002)
MSKTILVTGGAGFIGSAVVRHLINDTDHTVVNLDKLTYAGNLESLTPVSDSDRYSFEQVD
ICDAEEVKRVFNTHQPDIIMHLAAESHVDRSIDGPGEFIQTNVVGTYTLLEQARAYWSAL
EGDKKAGFKFHHISTDEVYGDLPHPDEVEPGTELPLFTETTPYAPSSPYSASKASSDQLV
RSWLRTYKLPTLVTNCSNNYGPYHFPEKLIPLVILNALAGKPLPVYGKGNQIRDWLYVED
HARALVVVALNGEIGETYNIGGHNEKQNIEVVQTICSILDEVKPKDSKYADQITYVQDRP
GHDMRYAIDASKIERELGWKPQETFESGIRKTVEWYLANEGWWKAVLDGSYQGERLGTGS