Protein Info for MIT1002_03516 in Alteromonas macleodii MIT1002

Annotation: Acetyl-coenzyme A synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 21 to 643 (623 residues), 997.8 bits, E=8.7e-305
• PF16177: ACAS_N
  • amino acids 24 to 81 (58 residues), 73.3 bits, 1.7e-24
• PF00501: AMP-binding
  • amino acids 84 to 468 (385 residues), 290.7 bits, E=2.3e-90
• PF13193: AMP-binding_C
  • amino acids 531 to 609 (79 residues), 89.1 bits, E=4.4e-29

Best Hits

Swiss-Prot: 72% identical to ACSA_PSEA6: Acetyl-coenzyme A synthetase (acsA) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 96% identity to amc:MADE_00711)

MetaCyc: 65% identical to acetyl-CoA synthetase (AMP-forming) (Escherichia coli K-12 substr. MG1655)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• 2-methylcitrate cycle I (5/5 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• chitin deacetylation (3/4 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• acrylate degradation II (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (6/10 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• β-alanine biosynthesis II (2/6 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (2/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.17

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Protein Sequence (648 amino acids)

>MIT1002_03516 Acetyl-coenzyme A synthetase (Alteromonas macleodii MIT1002)
MTASKTYPVPENILQSTHLTASQYDEMYKQSVEQPEAFWAEHASMLEWYQKPTKIKNTMF
GENDVSIKWFEDGELNASYNCIDRHLADNATKVAFHWEGDSPEDSQDITYQQVHYEVCKL
ANALKEMGVAKGDRVAIYMPMVPEAAYAMLACARIGAVHSVIFGGFSPNAIADRINDSNA
KVVITADEGRRAGRSIPLKANVDKALAGDACPSITHVLVHKLTGGDVDWNAKHDVWWHDA
VDGVSAQCEPEVMNAEDPLFILYTSGSTGTPKGVVHTTGGYLLYSAMTFKYAFDYKEDDV
YWCTADVGWITGHSYMVYGPMVNAASQVFFEGVPTYPDVKRIAQVVEKYKVNSLYTAPTA
IRALMAHGDIPAEGCDVSSLRLLGTVGEPINPEAWEWYHRVIGMSRCPIIDTWWQTETGG
HMILPLPGATELKPGSATRPFFGIQPALFDADGNELEGAAEGNLVIKDSWPSQARTVYGD
HQRFINTYFSAYKGVYFTGDGARCDEDGFYWITGRVDDVLNVSGHRLGTAEIESALVAHP
KVAEAAVVGFPHDIKGQGIYVYVTPNEGVEANEALTKELKAWVRQELSPIATPDMIQWSR
GLPKTRSGKIMRRILRKIAANEHEQLGDTSTLADPSVVDTLIEERLNK