Protein Info for MIT1002_03358 in Alteromonas macleodii MIT1002

Annotation: DNA polymerase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 767 PF03104: DNA_pol_B_exo1" amino acids 137 to 286 (150 residues), 59 bits, see alignment E=1e-19 PF00136: DNA_pol_B" amino acids 367 to 738 (372 residues), 118.1 bits, see alignment E=9e-38

Best Hits

KEGG orthology group: K02336, DNA polymerase II [EC: 2.7.7.7] (inferred from 82% identity to amc:MADE_01452)

Predicted SEED Role

"DNA polymerase II (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (767 amino acids)

>MIT1002_03358 DNA polymerase II (Alteromonas macleodii MIT1002)
MYVELWVKTPSSTARLISSPQYPTCFVSVDHANDLRNIAERISAKISISDDEFHTLEQVP
VATVKTATESHMHQLRQHAQESSIILYEADIRVADRYLMERFVYGAVEFLAPQDFDDTPQ
IVIENAQIRPCQFRTTFTSLSIDIECNEHEELFSIALAGEGLNVVLLLCPPHFVGQTLQG
QEDSAYELITVESEKVLLEAFFSYLTSYDPDIILGWNVKQFDMAVLERRARANSLKYAIG
RHGREASVRQWEDQTLVDVPGRCVIDGIEALKTMTYQFESFSLDHVSEELLGENKLIQAA
DKLEAIKRLYHSDPVALANYNFKDTELVNRIQDKTQFIDFLALRSILTGLDMSRPGGSVA
AFLNVYLPKLHRKSYVAGVRPENGGLASPGGYVMRSQPGLYKDVLVLDFKSLYPSIIRTF
KIDPLGLAEGLKSPDTAIEGFKGAVFSRESHFLPSIIENLWLQRDEAKKQNDAPRSQAIK
ILMNSFYGVLGSGGCPFYDPRLASSITLRGHEIMQTTAGWIEELGYTVIYGDTDSTFVHV
EGNDALGTPPETGLELASIINRKWTELLKSEFDIDCHLEIEFESHFSTFFMPTIRGSSEG
SKKRYAGMKGGELVFKGLENVRSDWTVLAKTFQYELYKKVFTGQPVEDYINSTIAAIKSG
EMDHALVYKKRLRKPLSSYTKSLPPHVKAARLADEYYKEQGLPLRYQFNTTIAYVITVQG
PQTAETATAPLQYDHYIEKQIQPIADSILPLIGLNFETIANDQLSLF