Protein Info for MIT1002_03279 in Alteromonas macleodii MIT1002

Annotation: Quinoprotein ethanol dehydrogenase precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 595 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 26 to 558 (533 residues), 783.5 bits, see alignment E=4.8e-240 PF13360: PQQ_2" amino acids 72 to 234 (163 residues), 73.4 bits, see alignment E=3.4e-24 amino acids 472 to 540 (69 residues), 29.5 bits, see alignment E=8.8e-11 PF01011: PQQ" amino acids 94 to 125 (32 residues), 27.1 bits, see alignment (E = 3.8e-10) amino acids 143 to 172 (30 residues), 23.1 bits, see alignment (E = 6.9e-09) amino acids 509 to 540 (32 residues), 20.4 bits, see alignment (E = 5e-08) PF13570: PQQ_3" amino acids 113 to 161 (49 residues), 22.5 bits, see alignment 1.9e-08 amino acids 482 to 522 (41 residues), 20.3 bits, see alignment 9.2e-08

Best Hits

Swiss-Prot: 52% identical to EXAA_PSEAE: Quinoprotein alcohol dehydrogenase (cytochrome c) (exaA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 88% identity to alt:ambt_01200)

MetaCyc: 52% identical to alcohol dehydrogenase (cytochrome c550) monomer (Pseudomonas aeruginosa)
RXN-11333 [EC: 1.1.2.8]

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.8

Use Curated BLAST to search for 1.1.2.8 or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (595 amino acids)

>MIT1002_03279 Quinoprotein ethanol dehydrogenase precursor (Alteromonas macleodii MIT1002)
MNNNMPIKKLALSMLVCASALTPVHAVVTDKDIQNDHVTTDDVVTYGMGLKAQRYSPLTA
INRDTVEEVRPVWAFSLGGEKQRGQESQPLVKDGVMYVTGSYSRVWAIDARTGEELWQYE
ARLPDGIMPCCDVINRGVALYDNLVIFGTLDAKLVALDKDTGKTVWKKKVEDYRAGYAIT
AAPIVVDGKVITGISGGEFGIVGKVYAYDAKSGKQLWVRPTVEGHMGYLWKDGKKTENGI
SGGAPGKTWPADLWKTGGAATWLGGTYDSDTGLLFFGTGNPAPWNSHLRPGDNYFSSSRL
AIDPNTGKIVWHFQTTPHDGWDFDGVNELISFDYQENGKTVKAAATADRNGFFYVLNREN
GDFIRGFPFVDKLNWAKGLDEKGRPIYVDENRPGSPAQSADGKQGAQVVARPAFLGGKNW
MPMAYSKDTELFYVPSNEWEMDIWNEPTSYKKGAAYLGAGFTIKSVNEDFIGVLKAIDPK
TGKTVWRYNNFSPLWGGVLTTGGGLVWTGNPEGYLMAFDDKTGEMVYKFQTGSGIVGSPV
TWEMDGEQYISVLSGWGGAVPLWGGEVAKRVKHFNQGGTVWTFKLPKRYQQVAKN