Protein Info for MIT1002_03212 in Alteromonas macleodii MIT1002

Annotation: putative type I restriction enzymeP M protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 522 TIGR00497: type I restriction-modification system, M subunit" amino acids 7 to 496 (490 residues), 441.4 bits, see alignment E=2.3e-136 PF12161: HsdM_N" amino acids 9 to 164 (156 residues), 81 bits, see alignment E=2.7e-26 PF02384: N6_Mtase" amino acids 176 to 479 (304 residues), 357.9 bits, see alignment E=1.1e-110 PF05175: MTS" amino acids 209 to 309 (101 residues), 27.4 bits, see alignment E=5e-10

Best Hits

Swiss-Prot: 39% identical to T1MD_MYCPN: Putative type I restriction enzyme MpnORFDP M protein (MPN_342) from Mycoplasma pneumoniae (strain ATCC 29342 / M129)

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 67% identity to sua:Saut_1346)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (522 amino acids)

>MIT1002_03212 putative type I restriction enzymeP M protein (Alteromonas macleodii MIT1002)
MVEQHKKALEKQLWNIANALRGNMSADEFRDYILGFIFYKYLSERMDIYADELLKEDCIK
FDEIDENAQQGKEYLDAIHEEAIDHLGYFLKPSELFHVLAEKGKNGEFILEQLTEVLNHI
EQSTMGTAAEDDFNGLFDDLDLTSNKLGKTEKAKNELISKILVHLDDIDFLHHETEIDVL
GDAYEYLIGQFASGAGKKAGEFYTPPMVSKLLAKLVTQGKDKLRSVYDPTCGSGSLLLRV
AKEVKDVGHFYGQEQNPSTYNLARMNMILHGVHYRRFDIQQDDTLEQPHHLEHKFEAVVA
NPPFSAKWSASQGFLTDERFQDYGKLAPASKADFAFVQHMIHQLDDNGTMAVVLPHGVLF
RGAAEGHIRKHLIKEKNYLDAVIGLPANIFYGTNIPTCILLIKKNRTNQDNILFIDGSQH
FGKAVNQNYLREEDVERILNAISSRKTEDKYSYVASISEVEEENDFNLNIPRYVDTFQED
APIDLEVISQRIREIEKSLKTTDSEIEVFCNELAIDSPIGKD