Protein Info for MIT1002_03139 in Alteromonas macleodii MIT1002
Annotation: Glutamate synthase [NADPH] large chain precursor
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 68% identical to GLTB_ECOLI: Glutamate synthase [NADPH] large chain (gltB) from Escherichia coli (strain K12)
KEGG orthology group: K00265, glutamate synthase (NADPH/NADH) large chain [EC: 1.4.1.13 1.4.1.14] (inferred from 60% identity to acb:A1S_3185)MetaCyc: 68% identical to glutamate synthase subunit GltB (Escherichia coli K-12 substr. MG1655)
Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of ammonia assimilation (plants) (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- L-glutamate biosynthesis IV (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14
Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1488 amino acids)
>MIT1002_03139 Glutamate synthase [NADPH] large chain precursor (Alteromonas macleodii MIT1002) MSLYNPNDSRDNCGFGLIAHTHGEASHELVTTAIHGLDRMQHRGGIAADGKTGDGCGLLL QKPDAFFHAIAEENGWKLSKKYGVGMIFLSQDPVLAQAAREVLNEELEKETLSVVGWREV PVDHSVLGELALTGVPQIEQVFVNAPAGWRKRDLERRLYMIRRRAEKRLEKDPDFYVACL SGLVTIYKGLVMPKDLPAFYHDLADERMQSAICVFHQRFSTNTLPRWPLAQPFRFLAHNG EINTIKGNRDWAMARAAKFATPLIPDLKDAAPFVNTEGSDSSSLDNMLELFLAGGMDLFR AMRLLVPPAYQNNKTMDDDLRAFYEFNSMHMEPWDGPAGIVLTNGRHVACNLDRNGLRPA RYVITKNGFITLASEVGIWDYEQADVIEKGRVGPGEMLAVDTYTGKIWRSTEIDEDLKAR HPYKEWLDKHIRRLTPIEKLDASLIGKRVFDDDAMAVYHKLHAYSYEEIQQVVKVLAKDG QEAVGSMGDDTPMAVMSSRQRTVYDYFRQQFAQVTNPPIDPLRENHVMSLATCIGREQNV FSETSGYADRVLFDSPVLMYTDLKQLREFNPDNYYSEVVELQYAPQEGLKKAIERVCNEV EMLVKNKRAAFVILSDRNIKQNRLVIPAAMAVGAVQRRLVEKSLRCDANVVVETASARDP HHFAVLIGLGATAVYPFLAYETIEQLCEKGELDISPMQATLNYRKGINKGLYKIMSKMGI STVASYRSSKLFEAVGINNEVMKLCFKGVTSRIQGAGFDDFQQDLINLNRIAWLKRKSVD HGGLLKYVHGGEYHAYNPDVVSTLQKAVVSGNYDDYQQYAKLVNERAPAHIRDLLAINPD CDPIDISEVEAPENLFPRFDTAAMSIGALSPEAHEALAIAMNRLGGQSNSGEGGEHPSRF GTEKNSKIKQVASGRFGVTPHYLVNANVIQIKVAQGAKPGEGGQLPGDKVNKYIAQLRFS VPGVTLISPPPHHDIYSIEDLAQLIFDLKQVNPTALISVKLVSEPGVGTIATGVAKAYAD LITVSGYDGGTGASPLTSVKYAGSPFELGLSETQQALIENGLRHKVRVQTDGGLKTGLDV VKAGILGAESFGFGTGPMVALGCKYLRICHLNNCATGVATQDQKLRDDHFIGLPDMVMNY FKFIAQEVREIMASMGIAKFDDLVGRTELLKVLDGITAKQNRLDLSPLLAQPKAGDHTRL FCSQTTNAPIDKGELNAQMLKDGQQAVVDGKSITLKYPIRNTDRSVGALLSGEIAKHHGN HDFEDTPIKVELTGTAGQSFGVWNAGGLHMHLEGDANDYVGKGMTGGKLVIHPPRNISYD AHDSAIMGNTCLYGATGGKLFAAGRAGERFGVRNSGAIAVVEGIGDNGCEYMTGGIVAVL GPVGVNFGAGMTGGFAYLMDDGDDLDNRVNAELVDVMPIEDKAILAEHLRGLINQHYEET GSEHSLSLLTDFAATLKRFKLIKPKTSDVKNLLGHISRSSAELRIQAQ