Protein Info for MIT1002_03130 in Alteromonas macleodii MIT1002

Annotation: 2-methylcitrate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00285: Citrate_synt
  • amino acids 9 to 356 (348 residues), 425.7 bits, E=7.4e-132
• TIGR01800: 2-methylcitrate synthase/citrate synthase II
  • amino acids 9 to 373 (365 residues), 520.9 bits, E=7.2e-161

Best Hits

Swiss-Prot: 77% identical to PRPC_SHEON: 2-methylcitrate synthase (prpC) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01659, 2-methylcitrate synthase [EC: 2.3.3.5] (inferred from 96% identity to amc:MADE_03371)

Predicted SEED Role

"2-methylcitrate synthase (EC 2.3.3.5)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 2.3.3.5)

MetaCyc Pathways

• 2-methylcitrate cycle I (5/5 steps found)
• 2-methylcitrate cycle II (5/6 steps found)

KEGG Metabolic Maps

• Propanoate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.5

Protein Sequence (374 amino acids)

>MIT1002_03130 2-methylcitrate synthase (Alteromonas macleodii MIT1002)
MAKQLSGAGLRGQVAGKTELSTVGKSGSGLTYRGYDVKDLAQNCQFEEVAYLILKGKLPN
QSELDAYKTKLRGMRGLPSALKEVLERIPKNAHPMDVLRTGCSMLGNLEMETSFDQQQDV
TDRMLACFPSIICYWYRFSHDGVRVDVETDDDSIGGHFLHMLHGEKPRELHEQVMHVSLI
LYAEHEFNASTFTARVCASTLSDMHSCVTGAIGSLRGPLHGGANEAAMEMIEGFTSADDA
EEKMMGMLERKEKIMGFGHAIYSESDPRNEIIKQWSEKLAADVGDDVLYPVSVRCEEVMW
REKKLFCNADFFHASAYNFMGIPTPLFTPIFVMSRLTGWAAHVMEQRADNRIIRPSAEYT
GEELRPVPAISERA