Protein Info for MIT1002_03017 in Alteromonas macleodii MIT1002

Annotation: preprotein translocase subunit SecA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 902 PF07517: SecA_DEAD" amino acids 7 to 402 (396 residues), 441 bits, see alignment E=5.8e-136 TIGR00963: preprotein translocase, SecA subunit" amino acids 28 to 817 (790 residues), 1152.2 bits, see alignment E=0 PF00270: DEAD" amino acids 100 to 216 (117 residues), 26.3 bits, see alignment E=1.7e-09 PF01043: SecA_PP_bind" amino acids 232 to 358 (127 residues), 134.2 bits, see alignment E=8.5e-43 PF21090: P-loop_SecA" amino acids 417 to 614 (198 residues), 319.7 bits, see alignment E=2.3e-99 PF07516: SecA_SW" amino acids 617 to 829 (213 residues), 250.6 bits, see alignment E=4.4e-78 PF02810: SEC-C" amino acids 882 to 900 (19 residues), 40 bits, see alignment (E = 8.3e-14)

Best Hits

Swiss-Prot: 73% identical to SECA_AERS4: Protein translocase subunit SecA (secA) from Aeromonas salmonicida (strain A449)

KEGG orthology group: K03070, preprotein translocase subunit SecA (inferred from 97% identity to amc:MADE_03250)

Predicted SEED Role

"Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (902 amino acids)

>MIT1002_03017 preprotein translocase subunit SecA (Alteromonas macleodii MIT1002)
MFSSILRKVFGSRNDRLLKKLRKNVDAINALEAEFEKLSDEALKAKTDEFKARIEKGETL
DDILVEAFATVREASKRVYGMRHFDVQMLGGQVLHQGKISEMRTGEGKTLTATLPTYLNA
LPGKGVHVVTVNDYLARRDAEWSNQLFTYLGMRVGCNVPGMSPQQKREAYQADVTYGTNN
EFGFDYLRDNMAFSPQDRVQRPLNYAVVDEVDSILIDEARTPLIISGQAEDSSELYRRIN
TIIPKLVQQEKEDEEGQEGDGDYTIDLKAKQIHLTERGQIHVEEILQQEGMLPEGESLFA
AGNISLLHHINAALRAHKLFAKDVDYIVKDDQIVIVDEHTGRTMEGRRWSEGLHQAVEAK
EGVRIQNENQTLASITFQNYFRLYNKLAGMTGTADTEAFEFNHIYGLETVVIPTNRPMQR
KDMADLIYLTAEEKYEAIVEDIKECVKRGQPTLVGTVSIENSELISRILKKSKIPHKVLN
AKFHEHEADIVAQAGKPGAVTIATNMAGRGTDIVLGGNWQAEVEKIENPTEAQIEKIKAE
WKESHDAVLASGGLHIIGTERHESRRIDNQLRGRSGRQGDPGSSRFYLSLDDALMRIFAS
EKMGNMMKRLGMERGEAIEHPWVTRAIENAQRKVEGRNFDIRKQLLEYDDVANDQRKVIY
EQRNELLDEGDISETIAAIREDVISSVVDEYIPPQSLEEMWDVSGLEERMRADFAVDLPI
KTWLENDDKLYEEKLRERILGEVVDAYKQKEAVVGEQVLRQFEKAVMLQNLDSHWKEHLA
AMDHLRQGIHLRGYAQKNPKQEYKRESFALFSQMLEALKVEVITILARVKVQAEEDVQKV
EEQRRQADDVPKNFEHEEANATPEEASDKVRTQVREGAKVGRNDPCPCGSGKKYKQCHGK
LK