Protein Info for MIT1002_02909 in Alteromonas macleodii MIT1002

Annotation: Choline dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 PF00732: GMC_oxred_N" amino acids 4 to 295 (292 residues), 200.4 bits, see alignment E=8.8e-63 TIGR01810: choline dehydrogenase" amino acids 5 to 532 (528 residues), 778.3 bits, see alignment E=1.9e-238 PF05199: GMC_oxred_C" amino acids 386 to 522 (137 residues), 143.8 bits, see alignment E=1.3e-45

Best Hits

Swiss-Prot: 68% identical to BETA_VIBVU: Oxygen-dependent choline dehydrogenase (betA) from Vibrio vulnificus (strain CMCP6)

KEGG orthology group: K00108, choline dehydrogenase [EC: 1.1.99.1] (inferred from 96% identity to amc:MADE_02642)

MetaCyc: 54% identical to choline dehydrogenase (Sinorhizobium meliloti Rm2011)
Choline dehydrogenase. [EC: 1.1.99.1]

Predicted SEED Role

"Choline dehydrogenase (EC 1.1.99.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.1.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.1

Use Curated BLAST to search for 1.1.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (550 amino acids)

>MIT1002_02909 Choline dehydrogenase (Alteromonas macleodii MIT1002)
MQTFDYIIVGAGSAGCVLANRLSENPKHEVLLLETGGSDKSIFIKMPTALSIPMNTDKYA
WQFNTEKEPYLNNREMHCPRGKVLGGSSSINGMVYVRGHAKDFDEWEAHGAEGWNYQACL
PYFQKAETWYKGNDAYRGGNGELGVNNGNEMKNPLYTAFIKAGEQAGYDITSDYNGKQQE
GFGPMHMTVKDGVRSSASREYLDPVKSRKNLTIVTGALVTKVVLEDKVAKGVEYVVNGKT
ETAATSNEVILSTGSIGSPHILQLSGIGDKDILEKAGVDVKHHLPGVGQNLQDHLEFYFQ
YKCKQPITLNRKLGLISKGLIGARWLLNRSGLGATNHFESCAFIRSKADVEWPDIQYHFL
PAAIRYDGKSAFDGDGFQVHVGHNKPKSRGSVTLQSANPTVPPKILFNYLQHPDDIEGFR
ACVRLTREIIAQSAFDDFRDGEIQPGEHIQTDEEIDAFVREAVESAYHPSCSCKMGEDEM
AVVDSQTNVHGIEGLRVVDSSIFPTIPNGNLNAPTIMVAEKAADIILGKPALPPQNVPVD
IHPHWQTKQR