Protein Info for MIT1002_02744 in Alteromonas macleodii MIT1002

Annotation: putative peptidoglycan biosynthesis protein MurJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 52 to 71 (20 residues), see Phobius details amino acids 92 to 122 (31 residues), see Phobius details amino acids 142 to 160 (19 residues), see Phobius details amino acids 167 to 189 (23 residues), see Phobius details amino acids 195 to 218 (24 residues), see Phobius details amino acids 245 to 274 (30 residues), see Phobius details amino acids 281 to 300 (20 residues), see Phobius details amino acids 320 to 342 (23 residues), see Phobius details amino acids 358 to 381 (24 residues), see Phobius details amino acids 395 to 412 (18 residues), see Phobius details amino acids 418 to 438 (21 residues), see Phobius details amino acids 450 to 470 (21 residues), see Phobius details amino acids 489 to 508 (20 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 4 to 509 (506 residues), 462.9 bits, see alignment E=6.8e-143 PF03023: MurJ" amino acids 30 to 470 (441 residues), 481.2 bits, see alignment E=1.4e-148

Best Hits

Swiss-Prot: 68% identical to MURJ_VIBCH: Probable lipid II flippase MurJ (murJ) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K03980, virulence factor (inferred from 94% identity to amc:MADE_03032)

MetaCyc: 45% identical to lipid II flippase MurJ (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-286

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (519 amino acids)

>MIT1002_02744 putative peptidoglycan biosynthesis protein MurJ (Alteromonas macleodii MIT1002)
MSRKLIKSGLIVSIMTLVSRVLGLVRDVVVAKLMGDGAAADVFFFANKIPNFLRRLFAEG
AFAQAFIPVLTEVHENDDKKQLREFVAKVSGTLGAIVFVVSIIGVIASPVLAALFGTGWF
VAWLEGGEAGDKFVLASTMLKITFPYLAFISLTGLAGAILNTLNKFAVAAFTPVLLNVCI
IACAIYLAPTLEQPAYALAWGVFIGGIVQFLFQLPFLFRAGLLVKPKWGWHDENVVKVRT
LMIPALFGVSVGQINLLFDTFIASFLVTGSISWLYYSDRLLEFPLGLFGIAIATVILPTL
SRNHVSNNPKAFAANIDWAFRMVCLLGIPAAVGLGVMARPILTVIFQRGAFTAETAIMAS
YSLTAYSFGLLSFMLVKILAPGFYSRQDTKTPVKFGIWCMVANMVFNLILAIPFGYVGLA
VATSMSATLNAALLYITLHKQGVFALSRTSVLFIARVVIASAAMGGLIYYRDQGLGFFEL
SLSAQMVEVGITISLSVILFVTTMVVLGMRPRHFRSGGD