Protein Info for MIT1002_02737 in Alteromonas macleodii MIT1002

Annotation: Tfp pilus assembly protein PilW

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 285 transmembrane" amino acids 12 to 36 (25 residues), see Phobius details PF07963: N_methyl" amino acids 7 to 31 (25 residues), 28.2 bits, see alignment (E = 9.9e-11) TIGR02532: prepilin-type N-terminal cleavage/methylation domain" amino acids 9 to 31 (23 residues), 21.6 bits, see alignment (E = 7.2e-09) PF16074: PilW" amino acids 155 to 276 (122 residues), 59.6 bits, see alignment E=3.3e-20

Best Hits

KEGG orthology group: K02672, type IV pilus assembly protein PilW (inferred from 89% identity to amc:MADE_03025)

Predicted SEED Role

"Type IV fimbrial biogenesis protein PilW" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (285 amino acids)

>MIT1002_02737 Tfp pilus assembly protein PilW (Alteromonas macleodii MIT1002)
MNARRHRPQQGFSLVELMITLTLGLVISGAIIQVLVSSSVTNKLNQAVAQVQESGRFIMA
RLSAEFYEVGRYDQIVADVDTSVDIVAEAAYVENHPIAVAGDFATNATLGSIQTSSGGSD
KLVVSLLSDEDCTGNQHGYGGLEFHVVNHYFVSGSTLKCTGYDGRVLRGLKVQTVSPLTV
TLLDNVESFQVQFGVSSSITNSQGQAVSYVTADEIEDLRALNQQVVSLRWALMLKSYQNE
VRQTQSQKYALLNEASKTMDTAHYYQVFSKTLALRNMKNFVRSSR