Protein Info for MIT1002_02692 in Alteromonas macleodii MIT1002

Annotation: Farnesyl diphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 PF00348: polyprenyl_synt" amino acids 31 to 268 (238 residues), 192.3 bits, see alignment E=4.3e-61

Best Hits

Swiss-Prot: 43% identical to ISPA_BACSU: Farnesyl diphosphate synthase (ispA) from Bacillus subtilis (strain 168)

KEGG orthology group: K00795, farnesyl diphosphate synthase [EC: 2.5.1.1 2.5.1.10] (inferred from 94% identity to amc:MADE_01426)

Predicted SEED Role

"Octaprenyl diphosphate synthase (EC 2.5.1.90) / Dimethylallyltransferase (EC 2.5.1.1) / (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) / Geranylgeranyl diphosphate synthase (EC 2.5.1.29)" in subsystem Archaeal lipids or Isoprenoid Biosynthesis (EC 2.5.1.1, EC 2.5.1.10, EC 2.5.1.29, EC 2.5.1.90)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.1, 2.5.1.10, 2.5.1.29, 2.5.1.90

Use Curated BLAST to search for 2.5.1.1 or 2.5.1.10 or 2.5.1.29 or 2.5.1.90

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (296 amino acids)

>MIT1002_02692 Farnesyl diphosphate synthase (Alteromonas macleodii MIT1002)
MNFSALHQQVKQQTDASLLTLIDELPDYAPRLKDAMRHALLAGGKRMRPLLVQVVGNTLD
VPKKDQMAISMAIECVHAYSLVHDDLPAMDDDDLRRGMPTCHIAFDEATAILAGDALQAL
AFTVLADAPLSTYAETKRSQLLSVLAKSAGYIGMCGGQAIDLASTGKTISLEELKQLHRL
KTGALLKACVEMVAIVSEGLLPQTKIDLMAYAADIGLAFQVQDDILDVEGSSEKLGKPAG
SDEALGKNTFPAKLGITGAKQELEMLHDNALQALARLPYNTDSLIAFSELLVKRDH