Protein Info for MIT1002_02648 in Alteromonas macleodii MIT1002

Annotation: High temperature protein G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 640 PF02518: HATPase_c" amino acids 32 to 186 (155 residues), 37.4 bits, see alignment E=4.7e-13 PF13589: HATPase_c_3" amino acids 33 to 151 (119 residues), 41.4 bits, see alignment E=2.2e-14 PF00183: HSP90" amino acids 224 to 636 (413 residues), 448.5 bits, see alignment E=6e-138

Best Hits

Swiss-Prot: 82% identical to HTPG_PSEA6: Chaperone protein HtpG (htpG) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K04079, molecular chaperone HtpG (inferred from 98% identity to amc:MADE_01104)

MetaCyc: 68% identical to chaperone protein HtpG (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein HtpG" in subsystem Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (640 amino acids)

>MIT1002_02648 High temperature protein G (Alteromonas macleodii MIT1002)
MAEAAHKETHGFQTEVKQLLHLMIHSLYSNKEIFLRELVSNAADAADKLRFRALENDSLY
ENDGELNVKLSVDKEANTVTITDNGIGMTRDEVIANLGTIAKSGTKDFFSKLSGDSAKDS
QLIGQFGVGFYSAFIVADKVTVRTRAAGADASQGVEWISDGEGEFTIAEINKPTRGTEIT
LHLREDEKEFADAWRLRSIVSKYSDHISIPVQMWKDEVPESEGPDGEKIEGQPGEWEVVN
KATALWTREKSEITDDEYNEFYKHISHDFADPLAWAHNKVEGKTEYTSLLYIPSKAPFDM
WNRDQSHGLKLYVQRVFIMDDAEQFMPTYLRFVKGLLDSNDLPLNVSREILQDNKITQAL
RQGCTKRVLQMLEKMAKNDNEKYQSFWNEFGNVLKEGPAEDFSNREKIAGLLRFSSTHGE
SDAQTVSLADYIERMKEGQDKIYYVTADSLQAAKSSPHLEIFRKKGIEVLLMGERIDEWL
MSHLTEFNEKQFVSIAKGNLDLGDLEDEESKKAQEEAEKQVEGVLERAKTALGDKVVDVK
FTHRLTDSPAVIVADDNGMTTQMMKLMQAAGQTVPDVKYHFELNPEHSLVKLLADTQDEE
LFNQWVGVLFDQAALSEQGSLKDPSSFVQNLNTLLMKLAK