Protein Info for MIT1002_02448 in Alteromonas macleodii MIT1002

Annotation: Polygalacturonase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 489 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF00295: Glyco_hydro_28" amino acids 236 to 470 (235 residues), 120.9 bits, see alignment E=6.4e-39

Best Hits

KEGG orthology group: None (inferred from 55% identity to sde:Sde_0953)

Predicted SEED Role

"Polygalacturonase (EC 3.2.1.15)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 3.2.1.15)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.15

Use Curated BLAST to search for 3.2.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (489 amino acids)

>MIT1002_02448 Polygalacturonase (Alteromonas macleodii MIT1002)
MMVRPNSPNARRHFLKSGLATTVAASGMAVGVPTFLSGCANVTETGRTQRQWDAEANLIK
GMIKRPTFAKREANVTDFLNENGSDFKQALHAAIDQVARQGGGKVVVPKGDWLCSGPIHL
QSNINLHIEAGATIRFSTNPDDYKPYVFTRWEGMELMGYSPLIYAFEQSNIAITGKGTLD
GSAAKDNWWPWKGKWKASTWGDDPVENQKYTRDTLQEMVENGTPVSDRVFENNCLRPPFI
QPYRCENVLIEGVTILRSPFWLVNPVLCKDVTVNDVYCKSFGPNSDGCDPESCTNVLISN
CTFDTGDDCIAIKSGRNADGRRVNVPCSNIVIEHCEMKAGHGGVVIGSEISGGVENLYAQ
HCTMSSPDLDRGIRIKTNSIRGGHLKNLNYRNIDIGTVKDAVVVNFFYEEGDAGNFPPLL
EDITIENLNVASANRAFVLRGYDHTPISGLTLNNITIKRALKDAVIENVAHLSINNVFIN
SQPFNKHGL