Protein Info for MIT1002_02399 in Alteromonas macleodii MIT1002

Annotation: 1,4-alpha-glucan branching enzyme GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01515: 1,4-alpha-glucan branching enzyme
  • amino acids 106 to 718 (613 residues), 840.3 bits, E=4.6e-257
• PF02922: CBM_48
  • amino acids 123 to 197 (75 residues), 67.8 bits, E=1.3e-22
• PF00128: Alpha-amylase
  • amino acids 270 to 341 (72 residues), 27.1 bits, E=4.6e-10
• PF02806: Alpha-amylase_C
  • amino acids 623 to 720 (98 residues), 67.2 bits, E=1.9e-22

Best Hits

Swiss-Prot: 72% identical to GLGB_PSEA6: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 87% identity to amc:MADE_01252)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

• glycogen biosynthesis I (from ADP-D-Glucose) (4/4 steps found)
• starch biosynthesis (6/10 steps found)
• glycogen biosynthesis II (from UDP-D-Glucose) (1/4 steps found)
• glycogen biosynthesis III (from α-maltose 1-phosphate) (2/8 steps found)

KEGG Metabolic Maps

• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.18

Use Curated BLAST to search for 2.4.1.18

Protein Sequence (724 amino acids)

>MIT1002_02399 1,4-alpha-glucan branching enzyme GlgB (Alteromonas macleodii MIT1002)
MQLAQQFEQVLCSQPFSHLGIVSNEQSSILRVWNPNASEITIKWGNPALKDTTVTSSDGL
FETPLPEKYQGEIYRVLAKGENAYIDPYQFTEQAYHAVHYIDSTPANLYEQAGAQLITLT
SPSGEAVKGVRFCVFAPNASTVSLIGDFNQWDGRLHPMEKTSMGYWVLFVPELGEGERYK
YHIKDAHGHDLPHKADPLGFSAEQYPSHASKIYNHNNYEWNDSAWMEKRKGNKYNSPMSI
YEVHLGSWKRPGIETDARYLTYKALADDLISYVSDMGYTHVELLPISEFPFDGSWGYQPV
GMFAPTSRFGNPDEFKYFVDKAHQAGIGVIIDWVPAHFPEDGHGLARFDGSCVYEYEDPR
KGWHPDWNSCIYDFGKDTVRQFLVANALFWLDKFHVDGLRVDAVASMLYLDYSRNDGEWI
PNVDGGNENYEAISLLKWMNEEVYKHFPDAMTIAEESTSFPKVSRPVFDGGLGFGFKWNM
GWMHDSLHYIAKDPAYRNYHHGDITFSMVYAFDENFVLPISHDEVVHGKGSMLHKMPGDE
WQQAANLRCYAGFMYGHPGKKLNFMGNELAQAREWNHDSSLDWHLLDYEKHKGIQALYKA
LNHLYTSTPALYEQDHEPGGFAWLDHNNADQSVVSFVRMSKDAKQKVYVVSNFTPVPRQN
FRIGVDDACTLTLALNTDDGAYWGSDYDVASTVNTQTIPWNERTHSAEITLPPLATVFYV
ANVD