Protein Info for MIT1002_02222 in Alteromonas macleodii MIT1002
Annotation: Acyl-coenzyme A dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 66% identical to FADE_ECOLI: Acyl-coenzyme A dehydrogenase (fadE) from Escherichia coli (strain K12)
KEGG orthology group: K06445, acyl-CoA dehydrogenase [EC: 1.3.99.-] (inferred from 98% identity to amc:MADE_02152)MetaCyc: 66% identical to acyl-CoA dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-17775 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-17796 [EC: 1.3.8.8, 1.3.8.7]; 1.3.8.- [EC: 1.3.8.8, 1.3.8.7]
Predicted SEED Role
"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)
MetaCyc Pathways
- oleate β-oxidation (32/35 steps found)
- fatty acid salvage (6/6 steps found)
- fatty acid β-oxidation I (generic) (6/7 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
- adipate degradation (4/5 steps found)
- 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
- pyruvate fermentation to butanol II (engineered) (4/6 steps found)
- pyruvate fermentation to hexanol (engineered) (7/11 steps found)
- (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
KEGG Metabolic Maps
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Brassinosteroid biosynthesis
- Butanoate metabolism
- Caprolactam degradation
- Fatty acid metabolism
- Geraniol degradation
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.2
Use Curated BLAST to search for 1.3.8.7 or 1.3.8.8 or 1.3.99.- or 1.3.99.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (820 amino acids)
>MIT1002_02222 Acyl-coenzyme A dehydrogenase (Alteromonas macleodii MIT1002) MADFIWFLLVVLTLAVASYQRTSLVTAVAMGAGVMILGTLFGDIGLLGWVVFLALTLPFT LANIRQQHISKKLLAFYRKVMPEMSSTEQEAIDAGTVWWDGDIFSGKPDWDKLHRIPKGR LTAEEQAFLDGPCDKVCSMVDEWQINHKDADLSPEIWQFLKEHKFFAMIIKKEYGGLEFS AYAQSRVLQKLAGVSAVLSSTVGVPNSLGPGELLQHYGTKEQQDHYLPRLAAGEEIPCFA LTGPEAGSDAGAIPDVGVVCKGEWNGEEVLGMRLTFNKRYITLAPVATVIGLAFKLQDPE GLLGDNKEPGITCALIPRDTKGLEIGRRHFPLNTPFQNGPIKGEDIFVPIDYIIGGPKMA GRGWRMLVECLSVGRCITLPSSAAGGAKSVSLATGAYARIRRQFRMPVGHMEGVEEMLAR IGGNAYLMDGVTRFSTVGVDLGEKPSVISAICKYHLTEKMRQIINDSMDVHGGKGVMLGP NNYLGRGYQGVPVSITVEGANILTRNMMIFGQGAMRCHPYVLKEIEAASIEDKKEALKAF DKAVFGHIGFAVANTVRSIWFSLTNGAFSSAPFTDETARYYKLLQGYSANLALLTDVSMG VLGGDLKRRERLSARLGDILSGLYMGSTTLKRFDEEGRLKEDLPLLHWAMQTTLHDIETA IDDFLANFPNRAIAAALRVMVIPFGRRIGKPSDKTEHAIAQMLQTPSTARSRLGYGQYLT REEGSLFGDLEQTLDDVLASEPIFERICKHKKAKLPFTRLDVLADEALAEGIINEEEANL LRKTEAGRLRTINVDDFDHEELVAKVNSNTTAKKRGGKAA