Protein Info for MIT1002_02202 in Alteromonas macleodii MIT1002

Annotation: Cyclic di-GMP phosphodiesterase Gmr

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 777 PF13185: GAF_2" amino acids 26 to 154 (129 residues), 39.8 bits, see alignment E=1e-13 amino acids 197 to 339 (143 residues), 57 bits, see alignment E=5.2e-19 PF01590: GAF" amino acids 27 to 156 (130 residues), 57.8 bits, see alignment E=3.7e-19 amino acids 199 to 338 (140 residues), 50.8 bits, see alignment E=5.3e-17 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 349 to 511 (163 residues), 149.6 bits, see alignment E=3.3e-48 PF00990: GGDEF" amino acids 351 to 508 (158 residues), 165.7 bits, see alignment E=1.6e-52 PF00563: EAL" amino acids 528 to 764 (237 residues), 254.4 bits, see alignment E=1.9e-79

Best Hits

KEGG orthology group: None (inferred from 74% identity to amc:MADE_02131)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (777 amino acids)

>MIT1002_02202 Cyclic di-GMP phosphodiesterase Gmr (Alteromonas macleodii MIT1002)
MRTPPTIVNEHERLAALNKLRVLDTPPEERFDRITRLASSIFDCRFASVTFVDDSRQWFK
SAINLDMTETERDVAFCAHAIAQDKTLVVPDTQEDPRFKSNPFVVGHPHIRFYAGVRLMV
EGFAVGTLCVSDNNPKIFNCVQIQQLEQLAKLIESELQRQEFNDLSTQLEEYQFKLAQTQ
KITRVRSAILEKVVNAESLHTVLHDIVNAIEAEYTDQICSILLLEDGKLKKGAAPSLPDF
YNDAIDGVEFGVGRGSCGNTAATNLPTIVEDISTHPFWTDWKDLALRAGVCSCWSQPIQG
ANGDAIGTFAIYHRERAVPTADEMRLIEQFAHIASIAIERELANQLIWKQANFDSLTGLP
NRNLMLDHLQLAIKAADRESDKVAVIFLDLDNFKDINDTLGHDIGDKLLIECARRIKQCL
RSDDTVSRLGGDEFVIILNGIKGLSSIELVVQKLLGEIVKPYSLELERVHTSASLGVTLY
PDDAKDVKGLLKNADQAMYGAKDLGKNNYQYYTHDMQTAAIKRMTLLNDLRYAVKNKELF
IEYQPIACFSSQKVMKAEALLRWQHPTKGRITPDEFIPLAEESGLIIDISNWVFNEVCAN
IKQWKAQNRQELQVSINTSPSHYFNLESNITQWLNLLLDQKISARSILLEVTENLLMEAN
EMVSKKLFQFRQAGVDIALDDFGTGYSSISYLKRFPTDFIKIDRSFVKSMTDVSNDKVLC
EAIIVMANKLGIKVVAEGVETQEQYDILKSMGCDYAQGYFIAKPLSKSDFESFWERH