Protein Info for MIT1002_02183 in Alteromonas macleodii MIT1002

Annotation: Alanine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 TIGR00518: alanine dehydrogenase" amino acids 1 to 374 (374 residues), 514 bits, see alignment E=1.1e-158 PF05222: AlaDh_PNT_N" amino acids 4 to 137 (134 residues), 175.1 bits, see alignment E=2.2e-55 PF01262: AlaDh_PNT_C" amino acids 141 to 353 (213 residues), 297.3 bits, see alignment E=1.5e-92 PF02826: 2-Hacid_dh_C" amino acids 169 to 268 (100 residues), 29.6 bits, see alignment E=1.1e-10 PF01488: Shikimate_DH" amino acids 169 to 270 (102 residues), 29.9 bits, see alignment E=1.3e-10 PF00070: Pyr_redox" amino acids 170 to 209 (40 residues), 23.8 bits, see alignment 1.3e-08

Best Hits

Swiss-Prot: 65% identical to DHA_HALED: Alanine dehydrogenase (ald) from Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)

KEGG orthology group: K00259, alanine dehydrogenase [EC: 1.4.1.1] (inferred from 94% identity to alt:ambt_08480)

MetaCyc: 58% identical to L-alanine dehydrogenase subunit (Bacillus subtilis)
Alanine dehydrogenase. [EC: 1.4.1.1]

Predicted SEED Role

"Alanine dehydrogenase (EC 1.4.1.1)" in subsystem Pyruvate Alanine Serine Interconversions (EC 1.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (377 amino acids)

>MIT1002_02183 Alanine dehydrogenase (Alteromonas macleodii MIT1002)
MLVGVPKEIKNHEYRVGLTPAAVKEFVSHGHSVLVETNAGEAIGFTNEMYVEAGAAIAST
AEQVFAEAEMIVKVKEPQPNECKMLRKGQTLYTYLHLAPDPTQTKLLVESGATCIAYETV
TDDRGGLPLLAPMSEVAGRMSVQAGAHYLEKAHGGSGTLLGGVPGVAPGKVLVIGGGVVG
TNAAKMALGLGADVTILDRSLPRLRQIDDIFNGQVKTVYSTVDAIEHYSSKADLVVGAVL
IPGAAAPKLLTREQIKAMKPGSVLVDVAIDQGGCFETSKATTHQDPVYVVDDVVHYCVAN
MPGGVARTSTMALNNATLPFGLALANKGPAKAMLEDKHLLNGLNVHEGKVTYKAVVDALG
EELGLTYTPAEVALKGE