Protein Info for MIT1002_02036 in Alteromonas macleodii MIT1002

Annotation: Ribosomal RNA large subunit methyltransferase K/L

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 PF02926: THUMP" amino acids 37 to 154 (118 residues), 44.6 bits, see alignment E=7.9e-15 PF01170: UPF0020" amino acids 163 to 372 (210 residues), 155.9 bits, see alignment E=5.4e-49 PF02475: Met_10" amino acids 451 to 603 (153 residues), 31.7 bits, see alignment E=6.5e-11 PF10672: Methyltrans_SAM" amino acids 473 to 650 (178 residues), 83.8 bits, see alignment E=6.1e-27 PF05175: MTS" amino acids 534 to 654 (121 residues), 31.1 bits, see alignment E=9e-11 PF03602: Cons_hypoth95" amino acids 534 to 656 (123 residues), 39.9 bits, see alignment E=1.8e-13 PF13847: Methyltransf_31" amino acids 535 to 655 (121 residues), 29.8 bits, see alignment E=2.5e-10

Best Hits

Swiss-Prot: 95% identical to RLMKL_ALTMD: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 95% identity to amc:MADE_02010)

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (698 amino acids)

>MIT1002_02036 Ribosomal RNA large subunit methyltransferase K/L (Alteromonas macleodii MIT1002)
MNTILVTTSRGLDELLKQEVLRLCPDAQVKQGPGTIQFEGSKEDAYKLCLWSRLANRVVW
VLASGKAGDADALYNTAMGIDWQMQMDSRHTLSVQFIGTNFAIKNTQFGAVRVKDAIVDS
FVEQGLPRPSVERKSPDISVYARLHRDNVILGIDLAGASLHQRAYRQETGDAPLKEHIAS
AMLMRSGWTENTDAPLVDLMCGSGTIAIEAAYIARNIAPGIKRMYWGFTKWLGHEEKLWD
DLVEQAISAQKPSCSGIYAGDFSRKMVAIAKANADFAGVFNDISFSQQDATKSSPPVATP
GYVVSNPPYGERLGELTSLIPLFSDWGKRFKEAWKGWHVSLLSSNRDLLRVLKLRATKDY
AMNNGKLECRLANYVLDEENTVQFSEDASNHEFANRLKKNLKRMKGWIKNANTNCYRIYD
ADLPDYNVAVDRYGDWLVVQEYAPPKTVSEDKARKRLQEVLLHLPAVTGVSPKQIALKVR
SQQKGTKQYEKINQTGDMMEVFENGARFLVNLTDYLDTGLFLDHRNTRQKVQALSQGKDV
LNLFSYTGSVSVFAAKGGAKSVTTVDMSNTYLEWAKKNVALNKLTAPHAFIQADCTTWLG
THKGKYDLIFIDPPSFSNSKRMQNTWDVQRDHVKMLTDAKACLKEQGIIIFSNNKRGFKL
DETAMSELGLSVDNITKDTIPEDFARKGNIHQCWVLAS