Protein Info for MIT1002_02035 in Alteromonas macleodii MIT1002

Annotation: Dihydroorotate dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01036: dihydroorotate dehydrogenase (fumarate)
  • amino acids 3 to 334 (332 residues), 445.3 bits, E=6.2e-138
• PF01180: DHO_dh
  • amino acids 46 to 334 (289 residues), 285.4 bits, E=2.4e-89

Best Hits

Swiss-Prot: 64% identical to PYRD_PSEA6: Dihydroorotate dehydrogenase (quinone) (pyrD) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 96% identity to amc:MADE_02009)

MetaCyc: 58% identical to dihydroorotate dehydrogenase, type 2 (Escherichia coli K-12 substr. MG1655)
Dihydroorotate dehydrogenase. [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.3.1 or 1.3.5.2 or 1.3.98.1

Protein Sequence (335 amino acids)

>MIT1002_02035 Dihydroorotate dehydrogenase (quinone) (Alteromonas macleodii MIT1002)
MQSLVQKFLLQCEPEKSHDFTINWLNKTQHTPLKWMYSTPTIKKPVTVAGMTFDNPVGLA
AGLDKNGDCIDAFASMGFGFVEIGTVTPRPQPGNPKPRLFRIPEKHAIINRMGFNNKGVD
HLVEQVKKAQFKGPIGINIGKNKDTEEANALDDYLICLNKVYPYASYVTINISSPNTPGL
RNLQYGEALDKLLVGLKAAQETLTQTHGKYVPLFIKIAPDLSDVEIDSIAASLINSKMDG
VIATNTTLSRDAVAGLQHAEEMGGLSGSVMTDMSLEVTRKLNIALDRAIPIIGVGGIDSP
EAAQARLNAGASLVQVYSAFIYQGPSLVKRIVNAL