Protein Info for MIT1002_02012 in Alteromonas macleodii MIT1002

Annotation: Pyruvate dehydrogenase E1 component subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00676: E1_dh
  • amino acids 58 to 350 (293 residues), 332.4 bits, E=1.1e-103

Best Hits

Swiss-Prot: 51% identical to ODBA_CAEEL: 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (bckd-1A) from Caenorhabditis elegans

KEGG orthology group: K00166, 2-oxoisovalerate dehydrogenase E1 component, alpha subunit [EC: 1.2.4.4] (inferred from 93% identity to amc:MADE_01954)

MetaCyc: 40% identical to 2-keto-isovalerate dehydrogenase component alpha subunit (Bacillus subtilis)
2-oxoisovalerate dehydrogenase (acylating). [EC: 1.2.1.25, 1.2.4.4]

Predicted SEED Role

"Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)

MetaCyc Pathways

• 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (3/3 steps found)
• L-leucine degradation I (5/6 steps found)
• L-isoleucine degradation I (4/6 steps found)
• L-threonine degradation V (1/2 steps found)
• L-valine degradation I (4/8 steps found)
• 2-oxobutanoate degradation I (1/4 steps found)
• superpathway of L-methionine salvage and degradation (6/16 steps found)
• anteiso-branched-chain fatty acid biosynthesis (18/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (18/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (18/34 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.4

Use Curated BLAST to search for 1.2.1.25 or 1.2.4.4

Protein Sequence (395 amino acids)

>MIT1002_02012 Pyruvate dehydrogenase E1 component subunit alpha (Alteromonas macleodii MIT1002)
MKDRNHLAEVKTTNKVEIITKGVVDIPMLQILSAEGELIEKAVEPDLSKEEALKIFNTMH
YIRVLDERMVGAQRQGRISFYLASTGEEAASVASAAALSDDDMVMSQYREQGALAYRGYT
TEQFMNQMFSNKDDPNKGRQMPIHYGDKPLNFMTISSPLGTQIPQASGYAYGQKMSGKDV
VTICYFGEGAASEGDFHAGLNMAAVLNCPVIFFCRNNGYAISTPAEEQFAGDGIASRGLG
YGIKTIRVDGNDVLAIYAATKEARRIAIEEKCPVLIEAMTYRLAAHSTSDDPTGYRSREE
EDKWRAKDPIARMAKWLESKGWFDEAENQKRVDKARQDVLAAMKSCEKTDVCAIEDIVED
VYDTAPWHLKEQLSELKAHIKKYPKMYPKTAGRVK