Protein Info for MIT1002_02000 in Alteromonas macleodii MIT1002

Annotation: putative succinyl-CoA:3-ketoacid coenzyme A transferase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 217 TIGR02428: 3-oxoacid CoA-transferase, B subunit" amino acids 3 to 207 (205 residues), 330.7 bits, see alignment E=1.4e-103 PF01144: CoA_trans" amino acids 5 to 200 (196 residues), 168.4 bits, see alignment E=1.6e-53 PF13336: AcetylCoA_hyd_C" amino acids 99 to 149 (51 residues), 23.8 bits, see alignment E=3.9e-09

Best Hits

Swiss-Prot: 61% identical to SCOB_MYCBO: Probable succinyl-CoA:3-ketoacid coenzyme A transferase subunit B (scoB) from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)

KEGG orthology group: K01029, 3-oxoacid CoA-transferase subunit B [EC: 2.8.3.5] (inferred from 96% identity to amc:MADE_01940)

MetaCyc: 57% identical to succinyl-CoA-transferase subunit B (Helicobacter pylori 26695)
3-oxoacid CoA-transferase. [EC: 2.8.3.5]

Predicted SEED Role

"Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5)" in subsystem Catechol branch of beta-ketoadipate pathway or Leucine Degradation and HMG-CoA Metabolism or Protocatechuate branch of beta-ketoadipate pathway or Serine-glyoxylate cycle (EC 2.8.3.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.5

Use Curated BLAST to search for 2.8.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (217 amino acids)

>MIT1002_02000 putative succinyl-CoA:3-ketoacid coenzyme A transferase subunit B (Alteromonas macleodii MIT1002)
MLSREQIAMRVAQEFKDGDYVNLGIGIPTLAANYVPDDISVMLQSENGLLGMGRYPTESE
IDADMINAGKETVTAITGASIFSSAESFAMIRGGHVDFTVLGAFEVDVSGNIASWMIPGK
LVKGMGGAMDLVAGAKNIIVTMTHTDKHGNPKLLGECTLPLTGVNCITRIITDLAVLSVE
DGAFVLQERAPGVSVDEIKEKTGGKLVVPENVSEMQF