Protein Info for MIT1002_01973 in Alteromonas macleodii MIT1002

Annotation: putative S-adenosyl-L-methionine-dependent methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 248 PF13489: Methyltransf_23" amino acids 31 to 146 (116 residues), 44.3 bits, see alignment E=4.2e-15 PF13847: Methyltransf_31" amino acids 44 to 152 (109 residues), 37.9 bits, see alignment E=3.5e-13 PF13649: Methyltransf_25" amino acids 46 to 134 (89 residues), 46.6 bits, see alignment E=1.1e-15 PF08242: Methyltransf_12" amino acids 46 to 137 (92 residues), 56 bits, see alignment E=1.4e-18 PF08241: Methyltransf_11" amino acids 47 to 137 (91 residues), 51.3 bits, see alignment E=3.9e-17

Best Hits

Swiss-Prot: 39% identical to CMOM_ECO57: tRNA 5-carboxymethoxyuridine methyltransferase (cmoM) from Escherichia coli O157:H7

KEGG orthology group: K06219, S-adenosylmethionine-dependent methyltransferase (inferred from 81% identity to amc:MADE_01900)

MetaCyc: 38% identical to tRNA cmo5U34 methyltransferase (Escherichia coli K-12 substr. MG1655)
2.1.1.M28 [EC: 2.1.1.M28]

Predicted SEED Role

"S-adenosylmethionine-dependent methyltransferase Functionally Coupled to the MukBEF Chromosome Partitioning Mechanism" in subsystem MukBEF Chromosome Condensation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.M28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (248 amino acids)

>MIT1002_01973 putative S-adenosyl-L-methionine-dependent methyltransferase (Alteromonas macleodii MIT1002)
MNNDRIFDGIAEKFSSNIYGTTKGKLRHTLLCDVLEPYLTTPVRAIEIGGGTGVMTAHIA
SKGHSVVLTDASKDVLKQAETLLADAPNITIRHQYLQQIEDLDSFDLVICHAVLEWLDAP
FDAIDFMYKNMKPGARLSLSFFNQDANLFANAIYGNFDYIAKGMKVKKQVRLNPKQPLSA
TRVVEHCESLGFKIIEKAGIRCFHDYMRDITHQSSKFDDLLALERQYHRQAPYLWLGKYF
QLILEKEK