Protein Info for MIT1002_01877 in Alteromonas macleodii MIT1002

Annotation: Diacylglycerol kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 542 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 30 to 48 (19 residues), see Phobius details amino acids 60 to 78 (19 residues), see Phobius details PF00782: DSPc" amino acids 99 to 228 (130 residues), 71.6 bits, see alignment E=8.5e-24 PF00781: DAGK_cat" amino acids 241 to 364 (124 residues), 97 bits, see alignment E=9.5e-32

Best Hits

KEGG orthology group: None (inferred from 85% identity to amc:MADE_01681)

Predicted SEED Role

"Methylglyoxal synthase (EC 4.2.3.3)" in subsystem Methylglyoxal Metabolism (EC 4.2.3.3)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.3.3

Use Curated BLAST to search for 4.2.3.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (542 amino acids)

>MIT1002_01877 Diacylglycerol kinase (Alteromonas macleodii MIT1002)
MKMVKYYVLGALVALALTLTVPVILLKVVFGWIAFSLIAVSSAYLLNYPSLFRKREDGSI
PFYIRWIFVPFLLGSWLYNEFARRTDKVPPLQKIEPHLFLACRMSGKHVSLLNENNIDAI
LDVTAEFDGLDWTAYQEDYRYLNVPVLDHTSPTPEQLVLAINWLNQQISEKKNVVVHCAL
GRGRSVLVVAAYLLAKNPTLSVDDALREINQIRQTARLNKRQLASLQQIRDGGLLSLQKN
LTLIVNPVAGGGKWEQYRDDVLSRLNEKFKVTVKETTPEVDGKALAEKAKQDGAHIIVAC
GGDGTLTEVASALVNTDVTMGIIPFGTANALSQVLHGYISKVMPISTACDIIIEGNTLAI
DTATCNDHVMLLVAAVGFEEKMISAADRDEKNVGGQFAYLKGLWNAISNNENMTFKVAKD
NQPAETLETPSFVIANAAPMTTALAQGAEPPDITDGKLDLTWLLPQPSSDKQFASLAELV
LSPAEAKKQSESIRHERASSITLTFDKPTAYAVDGEIYEGEKLEIKTCPRSLNVLTNFEE
KD