Protein Info for MIT1002_01848 in Alteromonas macleodii MIT1002

Annotation: D-alanyl-D-alanine carboxypeptidase DacC precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 394 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF00768: Peptidase_S11" amino acids 33 to 264 (232 residues), 297.5 bits, see alignment E=1e-92 PF13354: Beta-lactamase2" amino acids 43 to 227 (185 residues), 55.1 bits, see alignment E=1.1e-18 PF07943: PBP5_C" amino acids 284 to 374 (91 residues), 87.2 bits, see alignment E=9.8e-29

Best Hits

Swiss-Prot: 50% identical to DACC_ECOLI: D-alanyl-D-alanine carboxypeptidase DacC (dacC) from Escherichia coli (strain K12)

KEGG orthology group: K07258, D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5/6) [EC: 3.4.16.4] (inferred from 98% identity to amc:MADE_01652)

MetaCyc: 50% identical to D-alanyl-D-alanine carboxypeptidase DacC (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4 or 3.4.17.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (394 amino acids)

>MIT1002_01848 D-alanyl-D-alanine carboxypeptidase DacC precursor (Alteromonas macleodii MIT1002)
MLRIIQRAKPSLLSFAFLVIGTLLHTANAAVVTPPAPSVAAEGFLLTDFETGHVIASKNA
DMQLAPASLTKIMTIYVIGKELQAGNISLDDEVTISENAWAKKFPDSSKMFIEVGTQVSV
RDLLRGIVVQSGNDACVAMAEHVAGSESAFASMMNAHTASLGMSGSHWVNAHGLHDPDHY
TTPRDMAILSRALIAETPEMYKIYSEKEFTYNGIKQYNRNSLLWDKSLNVDGIKTGHTSD
AGYSLITSAEQGGMRLISVVMGTDSERARKVENKKLLKYGFRFYETLTPYQAGHSFVAHR
IYMGDRETVDLGINQSTPITIPRGQAANLEANFELDDKLEAPLAKGQVVGKLYLQLDGED
VASYPLVTLQEVKEGSFFNRMKDYIMLQLGFDDE