Protein Info for MIT1002_01740 in Alteromonas macleodii MIT1002

Annotation: putative deoxyribonuclease YcfH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 260 TIGR00010: hydrolase, TatD family" amino acids 2 to 252 (251 residues), 292.1 bits, see alignment E=1.8e-91 PF01026: TatD_DNase" amino acids 3 to 252 (250 residues), 275.1 bits, see alignment E=2.6e-86

Best Hits

Swiss-Prot: 50% identical to YCFH_ECOL6: Uncharacterized metal-dependent hydrolase YcfH (ycfH) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K03424, TatD DNase family protein [EC: 3.1.21.-] (inferred from 91% identity to amc:MADE_02328)

Predicted SEED Role

"Putative deoxyribonuclease YcfH" in subsystem YcfH

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.-

Use Curated BLAST to search for 3.1.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (260 amino acids)

>MIT1002_01740 putative deoxyribonuclease YcfH (Alteromonas macleodii MIT1002)
MFVDSHCHLDRLKQGPEELAETLNFARTRGVEHFLCVCVSVGDYDSMLAAVSQFEDVSVS
CGVHPLHQDEACSYEELLEKAQREEVVAIGETGLDYFYSPESKEVQLTSFVDHIKVANET
KKPLIIHTRDAREDTINLLRQHKAAHTKGVLHCFTESLDMALAAIEMDFYISISGIVTFN
SADELREVVKEIPLERLLIETDSPWLAPVPHRGKQNQPGYVVEVAEFIAELKGVTVKELA
EITTQNFYTLFSLAAKKKAA