Protein Info for MIT1002_01731 in Alteromonas macleodii MIT1002

Annotation: 3-oxoacyl-[acyl-carrier-protein] synthase 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 TIGR00747: 3-oxoacyl-[acyl-carrier-protein] synthase III" amino acids 4 to 321 (318 residues), 443.7 bits, see alignment E=1.8e-137 PF00108: Thiolase_N" amino acids 38 to 147 (110 residues), 33.6 bits, see alignment E=4.2e-12 PF08545: ACP_syn_III" amino acids 108 to 185 (78 residues), 97.7 bits, see alignment E=4.4e-32 PF08541: ACP_syn_III_C" amino acids 232 to 321 (90 residues), 119.2 bits, see alignment E=1.1e-38

Best Hits

Swiss-Prot: 89% identical to FABH_PSEA6: 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K00648, 3-oxoacyl-[acyl-carrier-protein] synthase III [EC: 2.3.1.180] (inferred from 95% identity to alt:ambt_09995)

MetaCyc: 64% identical to 3-oxoacyl-[acyl carrier protein] synthase 3 (Escherichia coli K-12 substr. MG1655)
[Acyl-carrier-protein] S-acetyltransferase. [EC: 2.3.1.38]; Beta-ketoacyl-acyl-carrier-protein synthase III. [EC: 2.3.1.38, 2.3.1.180]

Predicted SEED Role

"3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180)" (EC 2.3.1.180)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.180

Use Curated BLAST to search for 2.3.1.180 or 2.3.1.38

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (321 amino acids)

>MIT1002_01731 3-oxoacyl-[acyl-carrier-protein] synthase 3 (Alteromonas macleodii MIT1002)
MSKYSRFIGTGSYYPSDVRTNADLEQMVDTSDEWITDRTGIKERRIIGADETAATMGAEA
SKKAIEMAGIDPKSIDMIVCATTSGRYALPSTACEIQRILDIDGIPAFDVAAACAGYCYA
LSVADQYIKSGMAKRILVVGTDCLSRLINPEDRTMVILFGDAAGATIIEASDEPGILSTH
INASGSHGDLLYVGNPTRGDEQSVHENWGVMRGNEVFKVAVTKLSEVVEQTLAANGMQKS
DLDWLVPHQANFRIIKATAKKLNMSLDQVVLTLEQYGNTSAATVPTALDTAIRDGRIQRG
QHLLLEAFGGGFAWASALVRY