Protein Info for MIT1002_01713 in Alteromonas macleodii MIT1002

Annotation: Formate dehydrogenase H

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01701: oxidoreductase alpha (molybdopterin) subunit
  • amino acids 14 to 762 (749 residues), 958 bits, E=1.6e-292
• PF00384: Molybdopterin
  • amino acids 110 to 491 (382 residues), 91.4 bits, E=6.3e-30
• PF01568: Molydop_binding
  • amino acids 649 to 736 (88 residues), 35.3 bits, E=1e-12

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 91% identity to amc:MADE_02347)

Predicted SEED Role

"Putative formate dehydrogenase oxidoreductase protein" in subsystem Formate hydrogenase

MetaCyc Pathways

• formate oxidation to CO₂ (1/1 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• oxalate degradation VI (1/4 steps found)
• oxalate degradation III (1/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Protein Sequence (774 amino acids)

>MIT1002_01713 Formate dehydrogenase H (Alteromonas macleodii MIT1002)
MSDKRNIKTYNKPAGGWGALKSVTQSWIQSEKPLKNLRAMLKTNQDKGFDCPGCAWGESP
ESGLVKFCENGAKAVNWESTGRYVDPTFFSKYSVGSLKKHTDYWLESQGRLTHPMRYNAQ
ADHYEPVSWDDAFALIAKHLKGLHSPHQAEFYTSGRASNEAAYLYQLFVRAFGTNNFPDC
SNMCHEASGQGMKPTIGVGKGTVTFNDFAKADTIFVIGQNPGTNHPRMLEPLREAVRRGA
QVVCFNPLKERGLERFQNPQHPVEMLTNGSEPTNTAYFRPALGGDMAVMRGIAKWLLTWE
QEAKANNAPPVFDHDFINAHTNGMDDYLSAVEATSWEHIEAQSGLSKDEIHHAATMYRRG
ERVIMCWAMGITQHKHSVATVQEIVNVQLLRGNVGKPGAGLSPVRGHSNVQGDRTVGINE
VPPKALLDALENKFNFAVPRERGHNTIQAIQAMESGESKVFIGLGGNFAQATPDTPRTHA
ALSQCDLTVHIATKLNRSHLTTGKDALILPCLGRTEIDMQASGQQGVTVEDTFSMVHISY
GQLPPSSPELRSEPAIIAGIAKATLGNTPVDWDFLISDYDNIRELIADTIDGFKDFNTKL
AQPGGFHLGNAAGERRWLTPSGKAEFAANALPDSLLNADLVAKGEEPDLILQTLRSHDQY
NTTIYGMDDRYRNVFGARDVLFVNERDIKKLGFNDGDKVDITSMWNDGKTRQVKQFMLVA
YDIPQGQAAAYYPETNPLVPLESYGDGTFTPTSKFIAIKLSKSESDGRIAVSAV