Protein Info for MIT1002_01595 in Alteromonas macleodii MIT1002

Annotation: Glutamyl-tRNA(Gln) amidotransferase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 82% identity to amc:MADE_02494)

Predicted SEED Role

"Amidase"

MetaCyc Pathways

L-glutamate and L-glutamine biosynthesis (6/7 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
L-glutamine degradation I (1/1 steps found)
L-citrulline biosynthesis (6/8 steps found)
L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (421 amino acids)

>MIT1002_01595 Glutamyl-tRNA(Gln) amidotransferase subunit A (Alteromonas macleodii MIT1002)
MSSPFVLKPCDAKFTQAIKNLEIAFSLDGSVKDDAFSASGKALNQAKSQSKLPLSGLGLA
VKDLFHIEGLPTAAGNPDWLATHAIPKNTNRCVAKMLQAGATFKGKTITDELAYSLHGQN
KHYETLVNPVAPAHIPGGSSSGSAVAVSAHLADIGLGTDTGGSIRAPSSYQGLWGLRTTH
GVVACDNMVALAPSFDTVGWMTRDLNTLTKVANVCIDSATQSEISGSPRFGIAAHLFEQS
AHKSLCKTWLAALAENNDCITLSESQLDLNALQTAATFRTLQGNEIWQQHGEWIEYTQPD
IAKDIMLRLNWCKTITTQEVEQAKAQQSVVINHLNTLFEEIDVLIIPTTPGVAPRCDADE
TTLANDRNALLALTAIAGLAGLPQLHLPLFTLHNAPCGLSLVGKKGNDLALLALAKKLTT
R