Protein Info for MIT1002_01591 in Alteromonas macleodii MIT1002

Annotation: N-carbamoyl-L-amino acid hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01879: amidase, hydantoinase/carbamoylase family
  • amino acids 30 to 413 (384 residues), 394.1 bits, E=3.6e-122
• PF04389: Peptidase_M28
  • amino acids 68 to 183 (116 residues), 26.5 bits, E=7.5e-10
• PF01546: Peptidase_M20
  • amino acids 84 to 412 (329 residues), 86.7 bits, E=3.1e-28
• PF07687: M20_dimer
  • amino acids 219 to 317 (99 residues), 25 bits, E=2.2e-09

Best Hits

Swiss-Prot: 39% identical to AMAB2_GEOSE: N-carbamoyl-L-amino acid hydrolase (amaB) from Geobacillus stearothermophilus

KEGG orthology group: K02083, allantoate deiminase [EC: 3.5.3.9] (inferred from 93% identity to amc:MADE_02498)

Predicted SEED Role

"N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87)" in subsystem Hydantoin metabolism (EC 3.5.1.87)

MetaCyc Pathways

• allantoin degradation to ureidoglycolate II (ammonia producing) (1/4 steps found)
• allantoin degradation to glyoxylate II (1/5 steps found)
• allantoin degradation to glyoxylate III (1/5 steps found)
• allantoin degradation IV (anaerobic) (3/9 steps found)
• superpathway of allantoin degradation in plants (1/8 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.87 or 3.5.3.9

Protein Sequence (417 amino acids)

>MIT1002_01591 N-carbamoyl-L-amino acid hydrolase (Alteromonas macleodii MIT1002)
MGEFAEFAEFAELATQVMARCETLGTLSQDPACLDRRYLTEQHKLANQLASGWMIEAGMT
TWQDAVGNIWGRYTSSVPNAPRLILGSHLDTVPNGGKYDGMLGVVAAISLVAMFDGTQTK
FPFHIDIVGFCDEEGTRFGTTLLGSRALTGKWQEQWRHLKDENGVNLEQAMANFGLNFDA
VNSAAISRTDILGYIELHIEQGPVLEQENLPVGVVSAIAGAKRFIFSVEGMAGHAGTVPM
SMRKDALCASAEMLLAVESISLQRPGVVATVGKIENAPNGVNVISGRTRFSLDIRSEDDE
LRDSVLAEILQKFDTIAHARQITLTREQTHSAPAVHCDSQLKEALVRGVEESGIKPRVLA
SGAGHDAMAIADICPVAMLFTRCKGGLSHHPAESITIDDVAASLSVLYKTISKLKHT