Protein Info for MIT1002_01535 in Alteromonas macleodii MIT1002

Annotation: Maltodextrin phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 825 TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 16 to 818 (803 residues), 1179.1 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 104 to 818 (715 residues), 1082.2 bits, see alignment E=0

Best Hits

Swiss-Prot: 52% identical to PYG_DROME: Glycogen phosphorylase (GlyP) from Drosophila melanogaster

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 98% identity to amc:MADE_02552)

MetaCyc: 52% identical to glycogen phosphorylase b subunit (Drosophila melanogaster)
Phosphorylase. [EC: 2.4.1.1]

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (825 amino acids)

>MIT1002_01535 Maltodextrin phosphorylase (Alteromonas macleodii MIT1002)
MNAKATKTQPSPVIDKAAFKAAVIKHLHCTLGTDENKANNHAWWKATCAAIQEQVLEGLR
KTQKSHYLNDTRAVHYFSAEFLMGRLLSNNLQNFGLFDVASGALKELGVEISDILEEEPD
MALGNGGLGRLAACFIDSLATMELPAIGYGIHYEHGLFRQEIKSGEQIERPDSWRHYGNP
WEICRPESIQEVSLYGYVETKYGDNGRVLKEWHPGSIVKGVPWDIPVVGYEGKTVNVLRL
WQSEASGYFNWDVFNAGGYVDAQRENVSAETISKVLYPNDETEAGKELRLIQQYFFCSCS
LKDIIRRYKRAHGDDWSRFADQVVIQLNDTHPAISIPELMRILVDRAELGWDEAWGICTK
VFAYTNHTLLPEALEKWPARMIEKILPRHLEIIYEINHRFMAEVDKKWPGDNAMKAKLSI
IEEGNEKMVRMGHLSVIGSFAVNGVAEMHSRLVKSSLFPEFDELYPGKLTNVTNGITPRR
WLKACNPALSKLIDKKIGDDWPRDLDKLQGLAKFASNKTFQKQFMKVKLENKELLAEEIR
KSLDIEVDVNAIFDVQIKRLHEYKRQHLALLNIMTLYRRILENPDYDMHPRVFVFGAKAA
PGYKLAKDIIFAINKVADKINNDPRVNNKIKVAFLPNYRVSLAEKMIPAADVSEQISTAG
KEASGTGNMKLALNGAVTIGTLDGANVEIAEEVGDDNIFIFGLTVEEVNELKANGYNPHD
YYYKDPEIKAVLDWLETDYFTPGKPGALVSIKQSLLDNGDPYMVLADFRAYSDAQIAVDA
AYRDKERWAEMAIINTAKMGKFTSDRSIRDYVERVWKLSPCKIES