Protein Info for MIT1002_01513 in Alteromonas macleodii MIT1002

Annotation: Glutamyl-tRNA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 TIGR01035: glutamyl-tRNA reductase" amino acids 4 to 402 (399 residues), 447.6 bits, see alignment E=2.2e-138 PF05201: GlutR_N" amino acids 6 to 154 (149 residues), 185.8 bits, see alignment E=6.3e-59 PF01488: Shikimate_DH" amino acids 169 to 304 (136 residues), 154 bits, see alignment E=3.9e-49 PF00745: GlutR_dimer" amino acids 318 to 403 (86 residues), 60.9 bits, see alignment E=1.9e-20

Best Hits

Swiss-Prot: 66% identical to HEM1_PSEA6: Glutamyl-tRNA reductase (hemA) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K02492, glutamyl-tRNA reductase [EC: 1.2.1.70] (inferred from 89% identity to alt:ambt_11165)

MetaCyc: 54% identical to glutamyl-tRNA reductase (Escherichia coli K-12 substr. MG1655)
Glutamyl-tRNA reductase. [EC: 1.2.1.70]

Predicted SEED Role

"Glutamyl-tRNA reductase (EC 1.2.1.70)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (424 amino acids)

>MIT1002_01513 Glutamyl-tRNA reductase (Alteromonas macleodii MIT1002)
MTLLALGINHKTAPVALREKVAFTPDSLVEALASLKKLEGVDESVIVSTCNRTELYVNTQ
DDTGQKLLQWLSDFHHLDVEEIANNSYVLAQDEAVKHIMRVASGLDSLILGEPQILGQVK
QAFGDAKHSGMVSSEFDKLFQHTFSVAKRVRSETDIGANAVSVAYAAVQLAKHIFAELPK
RSVLLVGAGETIELVAQHLKEQGVSCLAVANRTVARAEALAETLDASVFTLSQVPEHLKD
FDIVISSTASQLPLIGKGMVEKALKQRKNMPMFLVDLAVPRDIESEVNELGDAYLYTVDD
LQHIVQKNLENREQAAKEAEKLIDKQAGDYMTWKQSQQSIDLVRQYRQKGMAQRDDIVEK
AKAQLAEGKNAETVLEEMAYKLTNTLLHPTTLALREAAMHDDPALSRWMGQALDLSDFSS
DDNK