Protein Info for MIT1002_01467 in Alteromonas macleodii MIT1002

Annotation: Carbamoyl-phosphate synthase large chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1052 (1051 residues), 1581 bits, E=0
• PF25596: CPSase_L_D1
  • amino acids 7 to 125 (119 residues), 166.5 bits, E=1.1e-52
  • amino acids 558 to 669 (112 residues), 165.9 bits, E=1.7e-52
• PF02786: CPSase_L_D2
  • amino acids 129 to 334 (206 residues), 242.3 bits, E=2.3e-75
  • amino acids 673 to 875 (203 residues), 129.9 bits, E=5.4e-41
• PF02787: CPSase_L_D3
  • amino acids 427 to 506 (80 residues), 90.8 bits, 2.5e-29
• PF02655: ATP-grasp_3
  • amino acids 677 to 845 (169 residues), 28.1 bits, E=1.1e-09
• PF02222: ATP-grasp
  • amino acids 679 to 845 (167 residues), 34.2 bits, E=1.1e-11
• PF07478: Dala_Dala_lig_C
  • amino acids 697 to 843 (147 residues), 25.4 bits, E=5.2e-09
• PF02142: MGS
  • amino acids 956 to 1041 (86 residues), 65.6 bits, E=2e-21

Best Hits

Swiss-Prot: 79% identical to CARB_ECO57: Carbamoyl-phosphate synthase large chain (carB) from Escherichia coli O157:H7

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 75% identity to abo:ABO_0318)

MetaCyc: 79% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• superpathway of arginine and polyamine biosynthesis (9/17 steps found)
• allantoin degradation IV (anaerobic) (3/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1073 amino acids)

>MIT1002_01467 Carbamoyl-phosphate synthase large chain (Alteromonas macleodii MIT1002)
MPKRTDIQSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPEM
ADATYIEPIHWEVVRKIIEKERPDAILPTMGGQTALNCALELDKHGVLEEFGCELIGATA
DAIDKAENRERFDKAMKNIGLECPRAEIAHSMDEAHDVLSRIGFPCIIRPSFTMGGTGGG
IAYNIDEFNEICHRGLDLSPTNELLIDESLLGWKEYEMEVVRDKADNCIIVCTIENFDPM
GVHTGDSITVAPAQTLTDKEFQLMRNAAMAVLREIGVETGGSNVQFGVDPKTGRVVIIEM
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELANDITGGLTPASFEPSIDYVVTKIP
RFNFEKFAGANDRLTTQMKSVGEVMAIGRNQQESLQKALRGLEVGATGLNPMVDLEDPES
RNKVVYELRQPGAERIWYIGDAFRMGMTVEEVFNLTNIDRWYLVQLEDLILLEAKVAELG
LPGIDADFMRALKRKGFSDARLAELTKVAESDIRETRRDFGISPVYKRVDTCAAEFSTST
AYMYSTYDEECEAAPTDKDKIMVLGGGPNRIGQGIEFDYCCVHAALSMREDGYETIMVNC
NPETVSTDYDTSDRLYFEPVTLEDVLEIVAKEQPKGVIVQYGGQTPLKLARALEAAGVPV
IGTSPEAIDRAEDRERFQQMVNKLNLKQPANATVTNVEQALTMAEEIGFPLVVRPSYVLG
GRAMEIVYDLKDLKRYLNEAVKVSNDAPVLLDRFLDDAIEVDIDAICDGKDVVIGGIMEH
IEQAGVHSGDSACSLPPHSLSKEIQDVMREQVKAMALELGVVGLMNTQFAIKDGEVYLIE
VNPRAARTVPFVSKATGVPLAKVAARVMAGVSLAEQGVTEEVIPPFYSVKEVVLPFAKFQ
GVDPLLGPEMRSTGEVMGVGDTFEEAYAKANLGAGAPLPKQGKALISVRDNDKPRMIALA
KALVEAGFSIEATRGTATELYDAGIMSTVVNKMSEGRPNIVDAIKNGEYAYLINTTEGRQ
AITDSVYIRREALLNKVTYTTTMNAAFATIRAKSADDRNKVASVQELHARLNH