Protein Info for MIT1002_01463 in Alteromonas macleodii MIT1002

Annotation: Acetyl-coenzyme A synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16177: ACAS_N
  • amino acids 3 to 56 (54 residues), 52.7 bits, 4.5e-18
• PF00501: AMP-binding
  • amino acids 64 to 444 (381 residues), 255.2 bits, E=1.4e-79
• PF13193: AMP-binding_C
  • amino acids 509 to 588 (80 residues), 91.4 bits, E=8.4e-30

Best Hits

Swiss-Prot: 58% identical to Y3568_PSEAE: Uncharacterized protein PA3568 (PA3568) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01908, propionyl-CoA synthetase [EC: 6.2.1.17] (inferred from 87% identity to amc:MADE_02248)

MetaCyc: 56% identical to propionate--CoA ligase (Ruegeria pomeroyi DSS-3)
Propionate--CoA ligase. [EC: 6.2.1.17]

Predicted SEED Role

"Propionate--CoA ligase (EC 6.2.1.17)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 6.2.1.17)

MetaCyc Pathways

• 2-methylcitrate cycle I (5/5 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• acrylate degradation II (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (6/10 steps found)
• β-alanine biosynthesis II (2/6 steps found)

KEGG Metabolic Maps

• Propanoate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.17

Protein Sequence (634 amino acids)

>MIT1002_01463 Acetyl-coenzyme A synthetase (Alteromonas macleodii MIT1002)
MTYKDEYIASTQHPDEFWLEQAQKLAWLQPPQKGCQPTDNGYFDWFRGGQLNTSYLALDH
HVKNGRGDKTALIYDSPVTQTTQHFTYKELLNRVAEFAGGLSRLGVTKGDRVIIYMPMIP
QAVIAMLACARLGAVHSVVFGGFAANELAVRIDDAKPVVIVTASCGIEGNKVLPYKPLVD
EAISLAGHKPQACVLFQRPQLIAELNEATDHDWLSLVEASVPASPVAVDATDPLYVLYTS
GTTGKPKGVVRDNGGHAVALHYSMGAIYGISENDIFWAASDVGWVVGHSYIVYGPLIKGA
TTVLYEGKPIGTPDAGAFWRMIESHKVNVLFAAPTAFRAIRKADPDAEFLSKYNTSSLRT
LFMAGERLDPPTYFWASEKVGVPVIDHWWQTETGWPICSNPMGLEPLATKPGSSSVPTPG
FNVKVLKGADEQQISGEKGAIAIKLPLPPGCLTTIWQDHQRFVDGYLREFEGYYSTGDNG
YVDEDGYVFIMGRTDDVINVAGHRLSTGEMEEVLAGHPAIAECSVIGIHDTLKGQLPVGL
VLLKDGVTIDEQALEQDLVQRVRDKIGPVACFKRAIVVNRLPKTRSGKILRKLLRQIAAN
ESVAVPSTIDDPSSVSEIETLMREKGLVNKPTAV