Protein Info for MIT1002_01458 in Alteromonas macleodii MIT1002

Annotation: Heat shock protein J

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 376 TIGR02349: chaperone protein DnaJ" amino acids 5 to 348 (344 residues), 469.1 bits, see alignment E=5.7e-145 PF00226: DnaJ" amino acids 5 to 67 (63 residues), 98.2 bits, see alignment E=3.5e-32 PF01556: DnaJ_C" amino acids 118 to 331 (214 residues), 188.7 bits, see alignment E=9.9e-60 PF00684: DnaJ_CXXCXGXG" amino acids 145 to 205 (61 residues), 66 bits, see alignment E=4.6e-22

Best Hits

Swiss-Prot: 84% identical to DNAJ_PSEA6: Chaperone protein DnaJ (dnaJ) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K03686, molecular chaperone DnaJ (inferred from 98% identity to amc:MADE_02252)

MetaCyc: 74% identical to chaperone protein DnaJ (Escherichia coli K-12 substr. MG1655)
1.8.4.-

Predicted SEED Role

"Chaperone protein DnaJ" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (376 amino acids)

>MIT1002_01458 Heat shock protein J (Alteromonas macleodii MIT1002)
MSKRDYYEVLGVDKSAGEREIKKAYKKLAMKYHPDRTQGDKGLEDKFKEIQEAYEILSDS
QKRAAYDQYGHAGVDPNRGGGGFGGGADFGDIFGDVFGDIFGGGRGGRQSRARQGSDLRY
NLELTLEEAVRGKSVDIRVPTLVECEVCDGSGAKKGSSPKTCPTCHGNGQVQMRQGFFAV
QQTCPTCSGKGKIIEDPCNSCHGHGRKEKTKTLNVKIPAGVDTGDRIRLSGEGEAGENGA
PPGDLYVQVHVREHDIFKRDGNNLYCEVPLSFTTAALGGELEVPTLDGKVKLKISPETQT
GRMFRLRGKGVKSVRSGAMGDLMCKVVVETPVNLSSRQKELLQELEESMGKASSKHSPKA
QGFFDGVKKFFDDLTN