Protein Info for MIT1002_01445 in Alteromonas macleodii MIT1002
Annotation: putative inorganic polyphosphate/ATP-NAD kinase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 63% identical to NADK_AERS4: NAD kinase (nadK) from Aeromonas salmonicida (strain A449)
KEGG orthology group: K00858, NAD+ kinase [EC: 2.7.1.23] (inferred from 97% identity to amc:MADE_02268)MetaCyc: 55% identical to NAD kinase (Escherichia coli K-12 substr. MG1655)
2.7.1.M29,2.7.1.23 [EC: 2.7.1.23, 2.7.1.M29]
Predicted SEED Role
"NAD kinase (EC 2.7.1.23)" in subsystem NAD and NADP cofactor biosynthesis global (EC 2.7.1.23)
MetaCyc Pathways
- NADP biosynthesis (1/1 steps found)
- NAD phosphorylation and transhydrogenation (1/2 steps found)
- cytosolic NADPH production (yeast) (3/5 steps found)
- mitochondrial NADPH production (yeast) (3/5 steps found)
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (5/8 steps found)
- NAD phosphorylation and dephosphorylation (1/3 steps found)
- NAD(P)/NADPH interconversion (3/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.23
Use Curated BLAST to search for 2.7.1.23 or 2.7.1.M29
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (291 amino acids)
>MIT1002_01445 putative inorganic polyphosphate/ATP-NAD kinase (Alteromonas macleodii MIT1002) MPYQTVALIGKPQHAATHDSLNILAEYLLAKGCKLLVEESIAEELETENFKSCNLVTIGK EADLAVVVGGDGSMLGAARVLARFDIHVVGVNRGNLGFLTDIHPDDIVQQLDLIFNGECV VEERFLLEVEVYRHEKLKSNNSAVNEVVLHHGKVAHMMEFEIYIDEQFVFSQRSDGLIVA TPTGSTAYSLSAGGPIIMPKLDALTLVPMFPHTLSSRPIVVDADSQVSMKVSKVNSDSLQ VSCDSHIVLPVLPGDEIRINKSVDKLHLVHPKGYSYFNVLRKKLGWGSKLY