Protein Info for MIT1002_01303 in Alteromonas macleodii MIT1002

Annotation: Efflux pump membrane transporter BepE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1038 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details amino acids 338 to 357 (20 residues), see Phobius details amino acids 364 to 384 (21 residues), see Phobius details amino acids 390 to 411 (22 residues), see Phobius details amino acids 435 to 455 (21 residues), see Phobius details amino acids 467 to 489 (23 residues), see Phobius details amino acids 527 to 547 (21 residues), see Phobius details amino acids 858 to 876 (19 residues), see Phobius details amino acids 883 to 903 (21 residues), see Phobius details amino acids 909 to 931 (23 residues), see Phobius details amino acids 958 to 979 (22 residues), see Phobius details amino acids 986 to 1011 (26 residues), see Phobius details PF00873: ACR_tran" amino acids 15 to 1012 (998 residues), 850 bits, see alignment E=2.4e-259 PF03176: MMPL" amino acids 298 to 491 (194 residues), 30 bits, see alignment E=2.7e-11

Best Hits

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 94% identity to amc:MADE_01699)

Predicted SEED Role

"RND multidrug efflux transporter; Acriflavin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1038 amino acids)

>MIT1002_01303 Efflux pump membrane transporter BepE (Alteromonas macleodii MIT1002)
MSDPNLSSSVSDLPSLSIRRPVLIVVLNLLIAIAGFAALSGLEVRELPDVDTPQVTVTAE
FPGASPETVDAEVTGRLEGAVARVSGVKDIYAQSEENSARVRVEFRPGVNLEDAANEVRE
SVSRIQRQLPEDVEQVSIIRADSDAQAVVSLAISSDTLDMETLTERVDTDVAPLFLSIPG
VADVMLDGERERVLRVSVDPLRLTSFGLSMSDIANALSLAPFDVPAGSIQSAEQALIVRA
DATSVSAEDVGNIVVSGDIRINDVASVYFGPADTTSVVRLDGTPVIGVGVIRQASSNTIE
ISDEVLAMVADLDKRFTDMHIVVTSDDAEFIRDSVKEVVVSLSLTVALVVVTLLVFIGSW
RATIVPALSIPVSLAGALAIIWGMGFSINILTLLALVLATGMIVDDAIVVSENIQRRRSM
GLGARAAAVVGTREVFFAVVATTAVLASVFIPIAFLPSTAGLLFREFGGVLAGAVIISSF
VALSLVPALTARLKVKEAKESGLFSKTFGRFGNACLRLYQASLLKALKYGWVVGVLSLAA
GGGAYILSQNIDNELLPSEDRGTIRIFARGPDGAGLNFMDRQAEKMEELLLPYVDNGTID
SIYTVVGSWDPNIVFITAPLKHWDDRDKSLQEVVNEIRPKLQQIPGAPGSAFGGNSLNLR
GQSGGLEIALTGDTYENIFEASQAFSRQIEDALPELGRPRISYDPTQPQMRVNIDRRRAE
ELGVPLTDIASTLRAAVNGDDIADLNVGDQSIPIMLQTNNRSTLDPSELTSLYVKSTDGN
LVPLSSVAFISEEGVAAELERQAQRRAIKLEMELPEDVALSNVVENIRNLAAESLPNGIG
LVFLGEAQTYEETSKQVALTYVLAFVIVLLVLAAQFESVNSAVVVMLTVPFGIAAAVYAL
FLTGTSINIYSQIGLVMLIGLLAKNAILLIEFADQLRDKGYDIYDAIVEAGRVRLRPIMM
TLVSTILGGLPLILSTGAGAEARNAIGWVVFGGLGIAVVFTLYLTPVLYLALARFTKPRA
DESAKLEKEMEEANAQHS