Protein Info for MIT1002_01287 in Alteromonas macleodii MIT1002

Annotation: Fatty acid oxidation complex subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 719 TIGR02440: fatty oxidation complex, alpha subunit FadJ" amino acids 17 to 718 (702 residues), 1114.9 bits, see alignment E=0 PF00378: ECH_1" amino acids 24 to 231 (208 residues), 151.6 bits, see alignment E=5.1e-48 PF16113: ECH_2" amino acids 37 to 238 (202 residues), 94.8 bits, see alignment E=1.5e-30 PF02737: 3HCDH_N" amino acids 325 to 504 (180 residues), 197.7 bits, see alignment E=3.3e-62 PF00725: 3HCDH" amino acids 507 to 600 (94 residues), 81.6 bits, see alignment E=9.7e-27 amino acids 630 to 714 (85 residues), 25.3 bits, see alignment E=3.6e-09

Best Hits

Swiss-Prot: 62% identical to FADJ_IDILO: Fatty acid oxidation complex subunit alpha (fadJ) from Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)

KEGG orthology group: K01782, 3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase / 3-hydroxybutyryl-CoA epimerase [EC: 1.1.1.35 4.2.1.17 5.1.2.3] (inferred from 97% identity to amc:MADE_02700)

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3, EC 5.3.3.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17, 5.1.2.3, 5.3.3.8

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3 or 5.3.3.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (719 amino acids)

>MIT1002_01287 Fatty acid oxidation complex subunit alpha (Alteromonas macleodii MIT1002)
MSQEQDMTNEVQPTSGAFTLTKQDNGVAILSMDVPGESMNTLKAEFGDEISAMLDDIERD
SSIKGVVLTSGKPSSFVAGADITMLAACKTAEDATTIAAGGQAIFDRIENMKATFVAAIH
GPALGGGLELALACHYRVCTDSPSTQVGLPEVQLGLLPGSGGTQRLPRLIGIQQAMKMML
TGSPARAKQAKKYGIVDEVVPHSVLLKVAEQFALKRKPEREAPQKSVMDKMLEKTGPGRN
MMFKKAREATFAKTKGNYPAPGYIIDVIETGMNEGMKAGLKAEAEAFGKLVMTPESFQLR
QIFFATTEMKKENGVEGVKPEKMKKVGVLGGGLMGGGIAYVTSTKAGVPVRIKDVRAEGI
ANAMKYSYDILNKKVKKRFMRNSEMQKQLALLTGTLDYSGYQDADIVVEAVFEDLDLKQK
MVADIEENCKESTIFASNTSSIPITQIAAKAARPENVIGLHYFSPVDKMPLAEVIAQDKT
SDQVISSTVEFAKKQGKTPVVVKDGAGFYVNRILAPYMNEAANLILDGEPIDHIDKSLVK
FGFPVGPVKLLDEVGIDVGTKIIPFLVEAFGERFTAPSAFDKVLADGRKGKKNQKGFYSY
EGKKPGKEVDESIYELLGLSPSAKLSEKEVAERCVLMMLNEAARCLDEGVIRNARDGDIG
AIFGIGFPPFLGGPFRYMDTLGIKHLVARLNHYATAVGDKFAPAEVLVKMAENDQKFYR