Protein Info for MIT1002_01257 in Alteromonas macleodii MIT1002

Annotation: Electron transport complex protein RnfA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 193 transmembrane" amino acids 6 to 28 (23 residues), see Phobius details amino acids 38 to 59 (22 residues), see Phobius details amino acids 70 to 88 (19 residues), see Phobius details amino acids 99 to 122 (24 residues), see Phobius details amino acids 134 to 153 (20 residues), see Phobius details amino acids 165 to 190 (26 residues), see Phobius details TIGR01943: electron transport complex, RnfABCDGE type, A subunit" amino acids 3 to 191 (189 residues), 288.2 bits, see alignment E=2.3e-90 PF02508: Rnf-Nqr" amino acids 6 to 190 (185 residues), 201.8 bits, see alignment E=4.4e-64

Best Hits

Swiss-Prot: 88% identical to RNFA_VIBTL: Ion-translocating oxidoreductase complex subunit A (rnfA) from Vibrio tasmaniensis (strain LGP32)

KEGG orthology group: K03617, electron transport complex protein RnfA (inferred from 98% identity to alt:ambt_12510)

MetaCyc: 56% identical to Rnf complex RnfA subunit (Acetobacterium woodii)
TRANS-RXN-276 [EC: 7.2.1.2]

Predicted SEED Role

"Electron transport complex protein RnfA" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (193 amino acids)

>MIT1002_01257 Electron transport complex protein RnfA (Alteromonas macleodii MIT1002)
MTEFLLLLIGTVLVNNFVLVKFLGLCPFMGVSGKLETAMGMSMATTFVLTLASASSYLVE
TYLLAPLGIGYLRTLAFILVIAVVVQFTEMVVHKTSPTLYRLLGIFLPLITTNCAVLGVA
LLNLTEQHNFMQSLIYGFGAAVGFSLVLVLFAAMRERLAAADVPVSFKGASIAMITAGLM
SLAFMGFSGLVKV