Protein Info for MIT1002_01128 in Alteromonas macleodii MIT1002

Annotation: Alanine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 67% identical to SYA_IDILO: Alanine--tRNA ligase (alaS) from Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 97% identity to amc:MADE_02846)

MetaCyc: 63% identical to alanine--tRNA ligase/DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
Alanine--tRNA ligase. [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; RXN-19726 [EC: 6.1.1.7, 3.1.1.96]; 3.1.1.- [EC: 6.1.1.7, 3.1.1.96]

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

tRNA charging (21/21 steps found)
superpathway of chorismate metabolism (42/59 steps found)
shinorine biosynthesis (3/8 steps found)
vanchrobactin biosynthesis (2/8 steps found)
enterobactin biosynthesis (4/11 steps found)
ergotamine biosynthesis (4/12 steps found)
(2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
bacillibactin biosynthesis (3/12 steps found)
superpathway of ergotamine biosynthesis (5/18 steps found)
sulfazecin biosynthesis (1/16 steps found)
phosalacine biosynthesis (1/25 steps found)
phosphinothricin tripeptide biosynthesis (1/25 steps found)
corallopyronin A biosynthesis (2/30 steps found)
colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.96 or 6.1.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (865 amino acids)

>MIT1002_01128 Alanine--tRNA ligase (Alteromonas macleodii MIT1002)
MTLSTADLRNKFIDYFKSHGHQAVASSSLVPADDPTLLFTNAGMNQFKDCFLGAEKRSYT
RAVSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKKEAIEFAWNFLTKEIG
LPKEKLLVTVYSEDDEAFDIWENHIGVPKEKIIRISTSDNFWSMGDTGPCGPCSEIFYDH
GAHIWGGPPGTPEEDGDRFIEIWNLVFMQFNKQADGTMEPLPKPSIDTGMGLERISAILQ
NVHSNYEIDLFQNLIKSAASIVGSEDLENKSLRVIADHIRSCSFLICDGVMPSNEGRGYV
LRRIIRRAVRHGYQLGANDIFFYKLVASLAKEMGEAYPELVDQLPVIEKVLRVEEEQFSK
TLARGMAILNDTLETLEGDTVPGDVVFKLYDTYGFPTDLTNDVAREHDLKIDEEGFEAAM
QAQRARAQQASNFGADYNSKLAIDHTTSFTGYTDVEGQANVVELIADNAFVEKLEDGQEG
VVVLDSTPFYAESGGQAGDKGVLRVANGEFIVTDTQKMGNAFAHKGIAKGSVSKGDTATA
AIDAANREAIKKNHSATHLLHAALREILGEHVTQKGSLVEAPRMRFDFSHFEAVTAEQLA
EIERRVNQEIRANHTLATELMDLDEAKASGAMALFGEKYDEKVRVVTMGPFSVELCGGTH
VTRTGDIGLFKITSEAGIASGVRRIEAVTGEHALEVVQTQQATLSSLSALLKTDSQNVLD
RVVSLQNQTKELEKALNSAKQKLASQQGADMLSNAVEINGVKVLIANLEGVEAKSLRSMM
DDIKNRIGEGIVVLGVANDAKVNLIAGVTKNLTSKVKAGELVNFVASQVGGKGGGRPDMA
QAGGDQPENLGQALDSVNAWLQDKL