Protein Info for MIT1002_01106 in Alteromonas macleodii MIT1002

Annotation: Chemotaxis protein CheA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 744 PF01627: Hpt" amino acids 8 to 107 (100 residues), 64.9 bits, see alignment E=1.3e-21 PF02895: H-kinase_dim" amino acids 357 to 416 (60 residues), 55.8 bits, see alignment 1.1e-18 PF02518: HATPase_c" amino acids 464 to 603 (140 residues), 67.6 bits, see alignment E=2.6e-22 PF01584: CheW" amino acids 610 to 737 (128 residues), 87.7 bits, see alignment E=1.1e-28

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 88% identity to amc:MADE_02867)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (744 amino acids)

>MIT1002_01106 Chemotaxis protein CheA (Alteromonas macleodii MIT1002)
MSFDVDEDILQDFLVEAGEILEQLQEQLVDLENNPEDADLLNAIFRGYHTVKGGAGFLSL
TELVEICHGAENVFDVMRNGQRTLTPELMDIILQATDVVVEMFERVKARDPLEPADAHLV
DLLHKLSRPESPDENIFGEQSAPVSEPEPAIEEPELVVEEPTAQAPSSGDSGGIDEITED
EFEALLDELHGSGAPGQSSAAKSEPAKAAAPAAASDNDDITDEEFEALLDDLHGKGKFGG
EEAAPKANAPAPAPVTPSEAGDEEITDDEFEALLDQLHGSGQGPTKQGAVSKAPEPPVDK
AATEAVQKAKQAAPAAPKATPAPSAAAPAKASTPAAPKAAPKKDEKKAAPAAPPAETTVR
VDTKRLDQIMNMVGELVLVRNRLISLGINSNDESMSKAIANLDVVTGDLQGAVMKTRMQP
IKKVFGRFPRVVRDLARSLKKEITLELVGEETDLDKNLVEALADPLVHLVRNSVDHGIEM
PDDRAAAGKPRMGTVQLSASQEGDHILLTIEDDGKGMDPEKLKEIAISRGVLDADAAARM
SDVEAFNLIFAPGFSTKTEISDISGRGVGMDVVKTKINQLNGTVNIDSQLGKGTRLDIKV
PLTLAILPTLMIVVGKQTFALPLGAVNEIINMDIKKTNTVDGQLTMIVRSKAIPLFFLGE
WLIRGPKNIDREKGHVVVVQIGTQQVGFVVDALIGQEEVVIKPLDALLQGTPGMAGATIT
SDGGIALILDIPSLLKRYARKPIK