Protein Info for MIT1002_01073 in Alteromonas macleodii MIT1002

Annotation: Pseudaminic acid synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 359 TIGR03586: pseudaminic acid synthase" amino acids 24 to 350 (327 residues), 466 bits, see alignment E=3.4e-144 PF03102: NeuB" amino acids 44 to 283 (240 residues), 285.4 bits, see alignment E=3.6e-89 PF08666: SAF" amino acids 298 to 356 (59 residues), 51.8 bits, see alignment E=9.9e-18

Best Hits

Swiss-Prot: 45% identical to PSEI_CAMJJ: Pseudaminic acid synthase (pseI) from Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)

KEGG orthology group: K01654, N-acetylneuraminate synthase [EC: 2.5.1.56] (inferred from 54% identity to bcg:BCG9842_B1724)

MetaCyc: 46% identical to pseudaminic acid synthase (Campylobacter jejuni)
RXN-10002 [EC: 2.5.1.97]

Predicted SEED Role

"N-acetylneuraminate synthase (EC 2.5.1.56)" in subsystem CMP-N-acetylneuraminate Biosynthesis or Sialic Acid Metabolism (EC 2.5.1.56)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.56 or 2.5.1.97

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (359 amino acids)

>MIT1002_01073 Pseudaminic acid synthase (Alteromonas macleodii MIT1002)
MKKLPTKPSAIALSRFTISDNNSPFIVAELSGNHNNSLADALELVDQAAAAGANGFKLQT
YTADTMTIKCSERDFLVKGTGKEWDERTLYDLYKEASTPWEWHQPIYERCKQKGMIPFST
PFDETAVDFLETLNNEIYKIASFELTDIRLLKKVAGTKKPMIVSTGMANESEVEKAVATI
RNEGNHNFVLLKCTSAYPAKYEDANLQTIPYFREKYGCHVGLSDHTLGLACPVIATAFGA
CVIEKHLKLEGQEGVDSHFSITPKEFKSMVEAINQGHASIGNIQTSLSDGEKASRNYRRS
IYATKDIKIGEIFTSENIRVIRPGFGLAPEHYESVIGKRCKCNIKRGTALSLSHIETIN