Protein Info for MIT1002_01011 in Alteromonas macleodii MIT1002

Annotation: Regulator of sigma E protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 99 to 122 (24 residues), see Phobius details amino acids 375 to 396 (22 residues), see Phobius details amino acids 425 to 446 (22 residues), see Phobius details TIGR00054: RIP metalloprotease RseP" amino acids 3 to 449 (447 residues), 448.5 bits, see alignment E=1.2e-138 PF02163: Peptidase_M50" amino acids 10 to 436 (427 residues), 257.5 bits, see alignment E=1.8e-80 PF17820: PDZ_6" amino acids 130 to 159 (30 residues), 29.6 bits, see alignment (E = 9.7e-11) amino acids 228 to 277 (50 residues), 35.9 bits, see alignment 1.1e-12 PF00595: PDZ" amino acids 202 to 276 (75 residues), 33.7 bits, see alignment E=7.8e-12 PF13180: PDZ_2" amino acids 213 to 287 (75 residues), 33.5 bits, see alignment E=8.4e-12

Best Hits

Swiss-Prot: 50% identical to Y2253_VIBCH: Putative zinc metalloprotease VC_2253 (VC_2253) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K11749, regulator of sigma E protease [EC: 3.4.24.-] (inferred from 96% identity to amc:MADE_01380)

Predicted SEED Role

"Membrane-associated zinc metalloprotease" in subsystem Sex pheromones in Enterococcus faecalis and other Firmicutes

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (450 amino acids)

>MIT1002_01011 Regulator of sigma E protease (Alteromonas macleodii MIT1002)
MLAFLWSLGAFIVALGILVAVHEWGHFYVARKCGVQVERFSIGFGKPIWRKTSKSGTEYV
IAMIPLGGYVRMLDGRIDDVPPELEDKAFNNKPVLQRMAVIAAGPGVNFIFAFFALWLMY
LVGLETVKPVVKSVEPESIAALAGVQAGDEIIKVGDRVTPDWEAVNLEIVSNIGAEKANV
TVKNSSNVEKELTFTLGSWNFDPDSESPLSSLGLTPYRPDATLTVGFVGEGSAAQQAGLK
PGDELLALNGAKLSSWERLVDVIVESPGENISLDIQRDGQQLTLDATIARRDTPQGQSGY
LGVSPTFEPWPEGYVFTHQYGIIEAIGKALDKTWRLMTLSVEMIGKLITGDVSVKNLSGP
ISIAQGAGTSAGYGLAYFLSFLALISVNLGIINLLPLPMLDGGHLMFFIVEWITGKPVPE
AVQEWGYRIGGVLLFMIMGIAIFNDIARIT