Protein Info for MIT1002_00970 in Alteromonas macleodii MIT1002

Annotation: UDP-glucose 4-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 PF00106: adh_short" amino acids 2 to 125 (124 residues), 35.4 bits, see alignment E=3.9e-12 PF04321: RmlD_sub_bind" amino acids 3 to 163 (161 residues), 46.3 bits, see alignment E=1.6e-15 PF08659: KR" amino acids 3 to 132 (130 residues), 33.2 bits, see alignment E=2.5e-11 PF05368: NmrA" amino acids 4 to 125 (122 residues), 26.6 bits, see alignment E=2e-09 PF01370: Epimerase" amino acids 4 to 263 (260 residues), 198.6 bits, see alignment E=5.7e-62 TIGR01179: UDP-glucose 4-epimerase GalE" amino acids 4 to 336 (333 residues), 470.1 bits, see alignment E=1.6e-145 PF02719: Polysacc_synt_2" amino acids 4 to 184 (181 residues), 68.4 bits, see alignment E=3.1e-22 PF01073: 3Beta_HSD" amino acids 5 to 159 (155 residues), 65 bits, see alignment E=3e-21 PF16363: GDP_Man_Dehyd" amino acids 5 to 326 (322 residues), 206.5 bits, see alignment E=3.9e-64 PF07993: NAD_binding_4" amino acids 6 to 190 (185 residues), 28.9 bits, see alignment E=3.1e-10 PF13460: NAD_binding_10" amino acids 8 to 175 (168 residues), 39.2 bits, see alignment E=3.4e-13

Best Hits

Swiss-Prot: 66% identical to GALE_BACSU: UDP-glucose 4-epimerase (galE) from Bacillus subtilis (strain 168)

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 97% identity to amc:MADE_03064)

MetaCyc: 66% identical to UDP-glucose 4-epimerase (Bacillus subtilis subtilis 168)
UDP-N-acetylglucosamine 4-epimerase. [EC: 5.1.3.7]; UDP-glucose 4-epimerase. [EC: 5.1.3.7, 5.1.3.2]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (340 amino acids)

>MIT1002_00970 UDP-glucose 4-epimerase (Alteromonas macleodii MIT1002)
MKTILVTGGAGYIGSHTVLQLLEQNYGVVVLDNLANASAESLRRVEALTGKSVTFVQGDI
RDTAVLDDIFSEHNIYAVIHFAGLKAVGESVQKPLSYYENNVYGTLTLCKAMQKHNVKNI
VFSSSATVYGDPASLPLREDMATGHPTNPYGMSKLMVEHMLSDLYVSDNEWNIVLLRYFN
PVGAHESGQIGEDPNGIPNNLMPYISQVATGKLEQLSVFGDDYDTVDGTGVRDYIHVVDL
ANGHLKALDRLNLNMGLDKYNLGTGQGYSVIEMIKAFEKGSGKTVSYKIAPRRSGDVAAC
YADPTKAATELNWHAEKGLEDMCADTWNWQSQNPMGYPKD