Protein Info for MIT1002_00951 in Alteromonas macleodii MIT1002

Annotation: Enolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01060: phosphopyruvate hydratase
  • amino acids 4 to 427 (424 residues), 686.2 bits, E=7.3e-211
• PF03952: Enolase_N
  • amino acids 4 to 134 (131 residues), 199.7 bits, E=1.9e-63
• PF00113: Enolase_C
  • amino acids 144 to 428 (285 residues), 469.2 bits, E=5.5e-145

Best Hits

Swiss-Prot: 99% identical to ENO_ALTMD: Enolase (eno) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K01689, enolase [EC: 4.2.1.11] (inferred from 99% identity to amc:MADE_03083)

MetaCyc: 81% identical to enolase (Escherichia coli K-12 substr. MG1655)
Phosphopyruvate hydratase. [EC: 4.2.1.11]

Predicted SEED Role

"Enolase (EC 4.2.1.11)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Serine-glyoxylate cycle (EC 4.2.1.11)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (16/17 steps found)
• gluconeogenesis I (13/13 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• glycolysis II (from fructose 6-phosphate) (11/11 steps found)
• glycolysis III (from glucose) (11/11 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• glycolysis I (from glucose 6-phosphate) (12/13 steps found)
• Entner-Doudoroff pathway I (9/9 steps found)
• homolactic fermentation (11/12 steps found)
• superpathway of anaerobic sucrose degradation (16/19 steps found)
• heterolactic fermentation (15/18 steps found)
• chorismate biosynthesis I (7/7 steps found)
• Rubisco shunt (9/10 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• Bifidobacterium shunt (12/15 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• glycolysis IV (8/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• gluconeogenesis III (9/12 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• glycolysis VI (from fructose) (8/11 steps found)
• superpathway of hexitol degradation (bacteria) (13/18 steps found)
• gallate biosynthesis (2/3 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of chorismate metabolism (42/59 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (6/9 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
• quinate degradation I (1/3 steps found)
• quinate degradation II (1/3 steps found)
• formaldehyde assimilation I (serine pathway) (8/13 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (4/9 steps found)
• glycerol degradation to butanol (9/16 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (9/35 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (14/42 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Glycolysis / Gluconeogenesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.11

Protein Sequence (430 amino acids)

>MIT1002_00951 Enolase (Alteromonas macleodii MIT1002)
MAKISRIIGREILDSRGNPTVEADVYLESGVMGRAAAPSGASTGSREALELRDGDKSRYL
GKGVTKAVAAVNDTIAPALIGKDALAQADIDGIMIDLDGTENKETLGANAILAVSLAVAK
AAAAEKGVALYEHIADLNGTSGQYSMPVPMMNIINGGEHADNNVDIQEFMVQPVGAKSFK
EALRMGAEIFHALKKVLSAKGLNTAVGDEGGFAPNLSSNAEALAVIVEAVENAGYKMNED
ITLALDCAASEFYKEGKYVLSGEDKSFDSEAFGDYLADLSSQYPIVSIEDGLDESDWDGW
ASLTKKIGDKVQLVGDDLFVTNTKILKRGIDNGIGNSILIKFNQIGSLTETLNAIKMAKD
AGFTAVISHRSGETEDATIADLAVGTAAGQIKTGSLCRSDRVAKYNQLLRIEEALGDAAI
YKGRSEIKGQ