Protein Info for MIT1002_00842 in Alteromonas macleodii MIT1002

Annotation: Periplasmic pH-dependent serine endoprotease DegQ precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR02037: peptidase Do" amino acids 41 to 452 (412 residues), 516.3 bits, see alignment E=3.4e-159 PF13365: Trypsin_2" amino acids 92 to 227 (136 residues), 122.4 bits, see alignment E=9.2e-39 PF00089: Trypsin" amino acids 93 to 254 (162 residues), 85 bits, see alignment E=2.5e-27 PF00595: PDZ" amino acids 264 to 345 (82 residues), 59.1 bits, see alignment E=1.6e-19 amino acids 373 to 442 (70 residues), 39.7 bits, see alignment E=1.8e-13 PF13180: PDZ_2" amino acids 282 to 355 (74 residues), 56.4 bits, see alignment E=1.1e-18 PF17820: PDZ_6" amino acids 292 to 344 (53 residues), 51 bits, see alignment 3.3e-17 amino acids 392 to 443 (52 residues), 31.2 bits, see alignment 5.1e-11

Best Hits

Swiss-Prot: 56% identical to DEGQ_ECOLI: Periplasmic pH-dependent serine endoprotease DegQ (degQ) from Escherichia coli (strain K12)

KEGG orthology group: K01362, [EC: 3.4.21.-] (inferred from 98% identity to amc:MADE_03178)

MetaCyc: 56% identical to periplasmic serine endoprotease (Escherichia coli K-12 substr. MG1655)
3.4.21.-

Predicted SEED Role

"Outer membrane stress sensor protease DegQ, serine protease" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (455 amino acids)

>MIT1002_00842 Periplasmic pH-dependent serine endoprotease DegQ precursor (Alteromonas macleodii MIT1002)
MKMSFRHCLLASAVVVSSLGFTASTQAALPFFSDKKDEVPSLAPMLEEATPAVVSIAVEG
TQTSTQRVPEMFRYFFGAPQEQVQERPFRGLGSGVIIDADKGYVVTNNHVVDNADEITVK
LTDGREFKAKKLGSDEQSDIALLKIEPDDLKALPLADSDALRVGDFVVAIGNPFGLSQTV
TSGIVSALGRSGLNIGGYEDFIQTDAAINRGNSGGALVNLHGELVGINTAIFGPNGGNVG
IGFAIPANMMKSLIDQIAEFGEVRRGLLGILGSDIDAGLAEAMNAEVNIGAFVSEVQPDS
AAEKGGLQAGDIITAINGRKLHSFQELRAKIASMGAGAEVELTVMRKGKKMNVDVVLDDA
TDTTVTAAQIHPALEGATLSNGTDDAGNAGVVVSDIERGAPAARIGLQSDDVIIGVNRVR
TSTVAEFRNALDEAKGVIALNVKRGNSTLYLVIRQ