Protein Info for MIT1002_00729 in Alteromonas macleodii MIT1002

Annotation: putative efflux pump membrane transporter TtgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1035 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 334 to 353 (20 residues), see Phobius details amino acids 360 to 380 (21 residues), see Phobius details amino acids 386 to 410 (25 residues), see Phobius details amino acids 431 to 451 (21 residues), see Phobius details amino acids 463 to 486 (24 residues), see Phobius details amino acids 525 to 543 (19 residues), see Phobius details amino acids 851 to 870 (20 residues), see Phobius details amino acids 878 to 898 (21 residues), see Phobius details amino acids 904 to 928 (25 residues), see Phobius details amino acids 950 to 971 (22 residues), see Phobius details amino acids 980 to 1001 (22 residues), see Phobius details PF00873: ACR_tran" amino acids 4 to 1006 (1003 residues), 988.1 bits, see alignment E=7.3e-301 PF03176: MMPL" amino acids 339 to 486 (148 residues), 24.6 bits, see alignment E=1.7e-09 amino acids 818 to 1010 (193 residues), 29.2 bits, see alignment E=6.8e-11

Best Hits

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 96% identity to amc:MADE_01735)

Predicted SEED Role

"RND multidrug efflux transporter; Acriflavin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1035 amino acids)

>MIT1002_00729 putative efflux pump membrane transporter TtgB (Alteromonas macleodii MIT1002)
MLLSDVSVKRPVFATVINLLLIIFGIVAFTMLSLREYPDIDPPIVSVSTTYTGASANIVE
TRITQLLEDRISGIEGIKNVTSTSRNGRSDITIEFKLSRDIDAAANDVRERVSRALNNLP
DQADPPEVSKANSDESAVVWYNLRSTNLNTMELTDYAERVLVDRLSIVDGVARVQIGGGR
RYAMKVFLDRNAMAARGITVNDVEQVIRTENVELPAGEVESTDRNFEVRVARTFLTPDDF
AALTVAIGDNGYLVRLGEIAHVELTAEDDETEFRGDGVNMIGLGIIKQSKANTLDVARAA
KAQIERIEATLPDNIFIVPSYDSSVFIEASIDEVYETLGIAMLMVVIVIYLFLGNIRATL
IPAVTVPVSIIAAFIVMYALGFSINLLTLLAMVLAIGLVVDDAIVVLENIYRRIEMGEPP
ILAAYRGAKEVGFAVIATTLVLISVFVPLVFLEGNIGRLFTEFALAIAAAVAFSSFTALT
LSPMMASKILKKRTRSSGFGSWMDKKFSKLENGYFNTLGKTLHQPILMLLMLAIAIVALV
QLSEKVASEFVPKEDRGNFFILMNAQEGASFESNAKNLEKIESILMPYRESGKINRLLVR
TPGFGGNAGIAIVGSADWDERDFSTFSLMDEISAKLNSVPDVRAFAIMRSGISGGGLGRP
VQFVLQGDTYENLVEWRDIVLQKAAQNPRLIRLDSDYKETSPQLLVNINRDRVADLGVSI
SDIGGTLEVMLGQRRVSTFLDRGEEYDVIMEGIEEDFRSPNSIENLYVRSSRTGELIPMD
NLLTFEEQATSAQLNRYNRMRAVTISANLADGYTLGQALEYLENIVSTELPDNVSIDYKG
ESQLYQEAGNSFVYVFMLALAVTYLILAAQFESWVHPLVIMLTVPLALVGAYIGLFFSDM
TINIYSQIGLVMLIGLAAKNGILIVEFANQLRDAGMEFEYALKRAAAQRLRPIVMTGFTT
VFSSLPLVLASGPGAESRMVIGMVIFSGVLVSAFMTLYVVPTAYSWLARNTGSPLQRTME
IETLEQSIPYKKGES