Protein Info for MIT1002_00728 in Alteromonas macleodii MIT1002

Annotation: Multidrug transporter MdtA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 349 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 42 to 346 (305 residues), 264.1 bits, see alignment E=7.7e-83 PF25917: BSH_RND" amino acids 67 to 189 (123 residues), 37.9 bits, see alignment E=4.5e-13 PF25973: BSH_CzcB" amino acids 68 to 193 (126 residues), 30.5 bits, see alignment E=9.9e-11 PF25944: Beta-barrel_RND" amino acids 198 to 272 (75 residues), 29.4 bits, see alignment E=3.2e-10 PF25954: Beta-barrel_RND_2" amino acids 200 to 271 (72 residues), 67.7 bits, see alignment E=3e-22 PF25967: RND-MFP_C" amino acids 277 to 335 (59 residues), 31.1 bits, see alignment E=6.5e-11 PF25989: YknX_C" amino acids 279 to 346 (68 residues), 60.9 bits, see alignment E=3e-20 PF25975: CzcB_C" amino acids 280 to 335 (56 residues), 29.4 bits, see alignment E=2.2e-10

Best Hits

KEGG orthology group: None (inferred from 86% identity to amc:MADE_01736)

MetaCyc: 34% identical to vibriobactin efflux pump periplasmic adaptor protein (Vibrio cholerae O1 biovar El Tor str. N16961)
TRANS-RXN-502

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (349 amino acids)

>MIT1002_00728 Multidrug transporter MdtA (Alteromonas macleodii MIT1002)
MSSRLSLSPLLLGVVLLAGVVVYLNMPEEESASNSIVRATPVKTALVSSQAFPITIESLG
TATANESVNITAQVTDTVKTINFEDGDKATEGELLVQLNNTEERARVEELKANIDEAKRQ
FTRISDLRQSNATSEQLLDEQQARVKALQAQLDVALAQLNDLQIRAPFSGLLGNREISIG
SLVQPGDTITTLDDISLIKVDFSIAESHLASVAKGQALSASSVAYPGEEFTGKISNIDTR
LDPISRSIRVRATIDNQDNRLRPGMLLTVIVEKRVLNTLVLPEKALVPVQDKQYVYVVKD
NVAHQTEVVIGERRPGLVQIVSGLNEGDEVITEGTLRVRDQSPVNVLNR