Protein Info for MIT1002_00718 in Alteromonas macleodii MIT1002

Annotation: serine/threonine protein kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 327 PF01636: APH" amino acids 34 to 260 (227 residues), 130.7 bits, see alignment E=4e-42

Best Hits

Swiss-Prot: 57% identical to SRKA_ECO57: Stress response kinase A (srkA) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 82% identity to alt:ambt_15105)

MetaCyc: 56% identical to stress response kinase A (Escherichia coli K-12 substr. MG1655)
2.7.11.d,2.7.11.11,2.7.12.1,2.7.11.12,2.7.11.17,2.7.11.1,2.7.11.13,2.7.11.22,2.7.11.21,2.7.11.34,2.7.11.31 [EC: 2.7.11.1, 2.7.11.11, 2.7.11.12, 2.7.11.13, 2.7.11.17, 2.7.11.21, 2.7.11.22, 2.7.11.31, 2.7.11.34, 2.7.11.d, 2.7.12.1]

Predicted SEED Role

"YihE protein, a ser/thr kinase implicated in LPS synthesis and Cpx signalling"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.11.1

Use Curated BLAST to search for 2.7.11.1 or 2.7.11.11 or 2.7.11.12 or 2.7.11.13 or 2.7.11.17 or 2.7.11.21 or 2.7.11.22 or 2.7.11.31 or 2.7.11.34 or 2.7.11.d or 2.7.12.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (327 amino acids)

>MIT1002_00718 serine/threonine protein kinase (Alteromonas macleodii MIT1002)
MTDFSFSGLSPDTILDALESQGIFLQSGLLALNSYENRVYQFLAEDGQRYVAKFYRPGRW
TDAQILEEHAFAAELIDNEIPLAAPMVINGNTLHHHTVAETDYRFALFPSVGGRQFENDN
LDQLEWMGRFIGRIHRVASAKGFTHRPSIDIESYLNEPKQVLAQSTLLPEHLKTAFFAIL
NPVIDATQKAYKPTSSIRLHGDCHPGNILWRDGPTFVDLDDCRMGPAIQDLWMMLSGDRQ
QQLLQLDTLVEAYEEFHSFDTAQLPLIEPLRAMRMVHYMAWLSRRWEDPAFPRAFPWFAE
DKYWEGQILALKEQLSALQEPPLKLGY