Protein Info for MIT1002_00700 in Alteromonas macleodii MIT1002

Annotation: Nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 969 PF04879: Molybdop_Fe4S4" amino acids 15 to 67 (53 residues), 49.4 bits, see alignment 7.1e-17 PF00384: Molybdopterin" amino acids 83 to 496 (414 residues), 242.8 bits, see alignment E=1.1e-75 PF01568: Molydop_binding" amino acids 615 to 728 (114 residues), 82.1 bits, see alignment E=6.2e-27 PF04324: Fer2_BFD" amino acids 911 to 959 (49 residues), 47.7 bits, see alignment 3.2e-16

Best Hits

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (969 amino acids)

>MIT1002_00700 Nitrate reductase (Alteromonas macleodii MIT1002)
MNMPTRQPDMMSEQLRQTTCPYCGVGCGVDISCNVSPSAITLDTVKGTPEHPANYGRLCV
KGTNLLETNDVNGRLLHPTMAGQSVDWDTVTDVIADKITSTIAQYGADAVAFYVSGQLLT
EDYYIANKLMKGYIGSANIDTNSRLCMSSAVAAYKRAFGEDVVPCDYTDLECTDLLVITG
SNAAWAHPVLFQRIQRAKLKNPNMKVIVIDPRKTETCTIADLHLPVKPGSDVALFNGLLS
YANAQGRIDKSAVDTYAEGLDETLKSANALTLDDVAEVCDLDVNSIKAFFDAFCKASSAI
TFYSMGVNQSSAGVDKAQAIINCHLAMDLIAKDGCGPFSITGQPNAMGGREVGGLANMLA
AHMDLENPEHISAVKTYWNAPVMPKGQGLKAVDLFNAIESGKVKFVWIMGTNPVVSMPNR
GQVERALSKCDMVVVSDIVESNDTLNYAHIALPATGWSEKDGTVTNSERRISRQRGILPP
PGSAKHDWQILCEVAGKMGFGEAFNFTHPSQIFCEYAGLTGYQNNGKRQLDLSPLQALSE
AQYNGLSPLQWPFQNVNKGVNTPTSDANFKANSKPSLTSKRPFENKQFSTPNGKAKLIPV
TYKAPLQITSKAYPFVVNSGRARDQWHTMTRTGKAAKLLAHMPSAYVHINPKDAAELGVE
SGDLVSLSSAVCKDAPVIYPVKADTDMRKGEVFIPIHWSAQWGSHSKLGALYASAVDPIS
GQPELKHAAVAIAPVHFATYGKLFVSSVNQSTLNSEFSPSYEDALKHACDYWNTTPLKST
EAISPVNNSDSALSVLNVASNKGRRDFTQGLIPLLPQNAVLLQFHHHTYSVCLALVDDTL
CFAAFLGDEPEKAERAPADAHSTAQVAPHSKNTWSVPNEWLASLLNTSVSTDIQRNLIRG
QVDDALLNGDVVCSCFEVREKTITHAIEEGCSSVAELGEALKCGTNCGSCKPALSQLIDK
HLVTEIKSV